Department of Food Technology, Şebinkarahisar School of Applied Sciences, Giresun University, 28400, Giresun, Turkey.
Department of Biology, Faculty of Science and Art, Giresun University, 28049, Giresun, Turkey.
Sci Rep. 2021 Jan 28;11(1):2534. doi: 10.1038/s41598-021-82132-4.
Cornelian cherry (Cornus mas L.) is a medicinal plant with antioxidant-rich fruits. Diniconazole, a broad-spectrum fungicide, is employed extensively. The present study was designed to evaluate the preventive efficiency of C. mas fruit extract (CME) against the toxic effects of diniconazole on a model organism, Allium cepa L. For this aim, physiological, cytogenetic and biochemical parameters as well as the meristematic cell damages were investigated in A. cepa treated with diniconazole and C. mas extract. A. cepa bulbs were divided into six groups which were treated with tap water, 0.5 g/L CME, 1.0 g/L CME, 100 mg/L diniconazole, 0.5 g/L CME + 100 mg/L diniconazole and 1.0 g/L CME + 100 mg/L diniconazole, respectively. Diniconazole application caused a significant reduction in germination percentage, root elongation and total weight gain. Mitotic index decreased, while chromosomal aberrations increased following diniconazole application. Diniconazole caused significant rises in malondialdehyde content and the total activities of superoxide dismutase and catalase enzymes. The meristematic cell damages induced by diniconazole were indistinct transmission tissue, epidermis cell deformation, thickening of the cortex cell wall and flattened cell nucleus. Aqueous C. mas extracts induced a dose-dependent prevention and amelioration in all damages arisen from diniconazole application.
欧李（Cornus mas L.）是一种药用植物，其果实富含抗氧化剂。多菌灵是一种广谱杀菌剂，被广泛使用。本研究旨在评估欧李果实提取物（CME）对多菌灵对模式生物洋葱（Allium cepa L.）的毒性作用的预防效率。为此，研究了在多菌灵和欧李提取物处理下，洋葱的生理、细胞遗传学和生化参数以及分生组织细胞损伤情况。将洋葱鳞茎分为六组，分别用自来水、0.5 g/L CME、1.0 g/L CME、100 mg/L 多菌灵、0.5 g/L CME+100 mg/L 多菌灵和 1.0 g/L CME+100 mg/L 多菌灵处理。多菌灵的应用导致发芽率、根伸长和总增重显著降低。有丝分裂指数下降，而染色体畸变增加。多菌灵处理导致丙二醛含量和超氧化物歧化酶和过氧化氢酶总活性显著升高。多菌灵引起的分生组织细胞损伤表现为不明显的传递组织、表皮细胞变形、皮层细胞壁增厚和平坦的细胞核。水提欧李提取物在多菌灵引起的所有损伤中具有剂量依赖性的预防和改善作用。
