Kroemer G, Blobel H, Brückler J
Zentralbl Bakteriol Orig A. 1977;237(2-3):183-8.
The conventional methods for the demonstration of coagulase, clumping-factor, fermentation of mannitol, protein A, alpha-, beta-, and delta-hemolysins and acid phosphatase were adapted to a microtitersystem (MTS). This led to a considerable saving in working-time and reagents. The biochemical reactions could be distinctly evaluated in the MTS (fig. 1). In all qualitative determinations there was complete agreement between the results obtained by MTS and the conventional procedures. In the quantitative endpoints there were only minor deviations (fig. 2,3,4). Thus, the MTS appeared to be a suitable procedure for the characterization of pathogenic staphylococci at a large scale.
用于检测凝固酶、凝聚因子、甘露醇发酵、蛋白A、α-、β-和δ-溶血素以及酸性磷酸酶的传统方法被应用于微量滴定系统（MTS）。这使得工作时间和试剂用量大幅节省。在MTS中可以清晰地评估生化反应（图1）。在所有定性测定中，MTS获得的结果与传统方法完全一致。在定量终点方面，仅有微小偏差（图2、3、4）。因此，MTS似乎是一种大规模鉴定致病性葡萄球菌的合适方法。
