Skalka B, Smola J, Pillich J
J Hyg Epidemiol Microbiol Immunol. 1979;23(4):407-16.
The authors have modified the one-plate method for the detection of staphylococcal hemolysins. They recommend to use in this method a prepurified form of staphylococcal beta-toxin and of streptococcal CAMP-factor instead of the exclusively beta-tonin-producing strain of Staphylococcus aureus and instead of the intensively CAMP-test positive Streptococcus agalactiae strain, respectively. The authors determined concurrently staphylococcal hemolysins, using a three-plate method in which alpha-antitoxin was employed, to ensure a better evidence of alpha-toxin. A total of 494 staphylococcal strains were examined by both methods. Of this number, 446 Staphylococcus aureus strains were of diverse host origin and 48 were coagulase-negative staphylococcal strains. On the basis of the various hemolytically active staphylococcal toxins, the authors recommend the suggested modification of the one-plate method for their routine detection.
作者对检测葡萄球菌溶血素的单平板法进行了改良。他们建议在此方法中分别使用预纯化形式的葡萄球菌β毒素和链球菌CAMP因子，而不是仅产生β毒素的金黄色葡萄球菌菌株以及CAMP试验强阳性的无乳链球菌菌株。作者同时采用使用α抗毒素的三板法来检测葡萄球菌溶血素，以更好地证实α毒素的存在。两种方法共检测了494株葡萄球菌菌株。其中，446株金黄色葡萄球菌菌株来自不同宿主，48株为凝固酶阴性葡萄球菌菌株。基于各种具有溶血活性的葡萄球菌毒素，作者推荐对单平板法进行所建议的改良用于常规检测。
