Yaremchuk O, Posokhova K, Lykhatskyi P, Letniak N, Kulitska M, Kuzmak I, Lisnychuk N, Delibashvili D
1I. Horbachevsky Ternopil National Medical University, Ukraine.
2Tbilisi State Medical University, Georgia.
Georgian Med News. 2020 Feb(299):120-125.
The aim of the study is to investigate the processes of apoptosis and the level of formation of reactive oxygen species (ROS) in blood cells of BALB/c female mice in experimental antiphospholipid syndrome (APS). The content of living, apoptotic and necrotic cells in blood leukocytes was evaluated. Apoptosis in cells was detected using Annexin V conjugated to GFP and propidium iodide. ROS in blood leukocytes were detected using 2',7'-dichlorofluorescein diacetate. The redistribution between the leukocytes populations was assessed by the magnitude of direct (FS) and lateral (SS) light scattering on the flow cytofluorimeter. It was established that the APS reduced the viability of blood leukocytes compare to the control. It is likely that their death is due partly to the activation of apoptosis. In the animals with APS a redistribution between the two main types of leukocytes (granulocytes and agranulocytes) was found out. An increase in the number of granulocytes in the blood was evidenced in cases of APS. It was established that the basal level of ROS production in granulocytes reduced by 27% and in agranulocytes - by 19% compare to the control. Thus, taking into account the attained results it can be argued that in the pathobiochemical mechanisms of APS development the enhanced activation of apoptosis and deficient formation of ROS is significant.
本研究的目的是调查实验性抗磷脂综合征（APS）中BALB/c雌性小鼠血细胞的凋亡过程和活性氧（ROS）的生成水平。评估了血液白细胞中活细胞、凋亡细胞和坏死细胞的含量。使用与绿色荧光蛋白（GFP）偶联的膜联蛋白V和碘化丙啶检测细胞凋亡。使用二乙酸2',7'-二氯荧光素检测血液白细胞中的ROS。通过流式细胞荧光仪上的前向（FS）和侧向（SS）光散射强度评估白细胞群体之间的重新分布。结果表明，与对照组相比，APS降低了血液白细胞的活力。它们的死亡可能部分归因于凋亡的激活。在患有APS的动物中，发现两种主要类型白细胞（粒细胞和无粒细胞）之间存在重新分布。APS病例中血液粒细胞数量增加得到证实。结果表明，与对照组相比，粒细胞中ROS产生的基础水平降低了27%，无粒细胞中降低了19%。因此，考虑到所获得的结果，可以认为在APS发展的病理生化机制中，凋亡的增强激活和ROS生成不足具有重要意义。
