KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.
PULSE MEDICA, MEDICAL CENTER, KHARKIV, UKRAINE.
Wiad Lek. 2022;75(9 pt 2):2270-2274. doi: 10.36740/WLek202209216.
The aim: To assess reactive oxygen species production by leukocytes and their viability in rats with chronic colitis.
Materials and methods: Reactive oxygen species production was estimated in leukocytes, isolated from rats with Dextran Sulfate Sodium-induced chronic colitis and control rats, by flow cytometry using the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate. Leukocyte viability and apoptosis stages were assessed by flow cytometry using annexin V and 7-aminoactinomycin D staining. White blood cell counting was carried out with using Hematology Analyzer.
Results: The increased fluorescence intensity of 2',7'-dichlorofluorescein in viable leukocytes by 36.7% was revealed in rats with chronic colitis compared control rats. A significant decrease in the percentage of viable cells and an increase in apoptotic cells were found compared to intact animals. Leukocytes, granulocytes, monocytes, lymphocytes counts in blood of experimental group animals were significantly higher compared to control those.
Conclusions: Our findings indicate that Dextran Sulfate Sodium-induced chronic colitis increases an intracellular production of reactive oxygen species by leukocytes. Despite of increased leukopoiesis it reduces viability of white blood cells and promotes their apoptosis via stimulation of oxidative stress.
评估白细胞产生的活性氧物质及其在慢性结肠炎大鼠中的活力。
通过使用荧光探针 2'，7'-二氯二氢荧光素二乙酸酯，通过流式细胞术评估来自葡聚糖硫酸钠诱导的慢性结肠炎大鼠和对照大鼠的白细胞中活性氧物质的产生。通过使用 Annexin V 和 7-氨基放线菌素 D 染色通过流式细胞术评估白细胞活力和凋亡阶段。使用血液学分析仪进行白细胞计数。
与对照组相比，慢性结肠炎大鼠白细胞中 2'，7'-二氯荧光素的荧光强度增加了 36.7%。与完整动物相比，发现活细胞的百分比显著降低，凋亡细胞增加。实验组动物血液中的白细胞、粒细胞、单核细胞、淋巴细胞计数明显高于对照组。
我们的研究结果表明，葡聚糖硫酸钠诱导的慢性结肠炎增加了白细胞内活性氧物质的产生。尽管白细胞增多，但通过刺激氧化应激，它降低了白细胞的活力并促进其凋亡。
