Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
J Agric Food Chem. 2020 Apr 29;68(17):4769-4787. doi: 10.1021/acs.jafc.0c00258. Epub 2020 Apr 16.
Ochratoxin A (OTA) is a class of mycotoxin that are mainly produced by Aspergillus and Penicillium and widely found in plant origin food. OTA-contaminated foods can cause serious harm to animals and humans, while high stability of OTA makes it difficult to remove in conventional food processing. Thus, sensitive and rapid detection of OTA undoubtedly plays an important role in OTA prevention and control. In this paper, the conventional and novel methods of OTA at home and abroad are summarized and compared. The latest research progress and related applications of novel OTA electrochemical biosensors are mainly described with a new perspective. We innovatively divided the recognition element into single and combined recognition elements. Specifically, signal amplification technologies applied to the OTA electrochemical aptasensor are proposed. Furthermore, summary of the current limitations and future challenges in OTA analysis is included, which provide reference for the further research and applications.
赭曲霉毒素 A（OTA）是一类主要由曲霉属和青霉属产生的真菌毒素，广泛存在于植物源性食品中。OTA 污染的食品会对动物和人类造成严重危害，而 OTA 的高稳定性使得其在常规食品加工中难以去除。因此，OTA 的灵敏、快速检测无疑在 OTA 防控中起着重要作用。本文综述了国内外 OTA 的常规和新型检测方法，并进行了比较。主要从新的视角描述了新型 OTA 电化学生物传感器的最新研究进展及相关应用。我们创新性地将识别元件分为单一和组合识别元件。具体来说，提出了应用于 OTA 电化学适体传感器的信号放大技术。此外，还对 OTA 分析的当前局限性和未来挑战进行了总结，为进一步的研究和应用提供了参考。
