J Biomed Nanotechnol. 2020 Aug 1;16(8):1296-1303. doi: 10.1166/jbn.2020.2959.
Ochratoxin is teratogenic, carcinogenic and immunotoxic to humans. It contains ochratoxin in many foods, so the detection of ochratoxin in food is particularly important. In this paper, gold nanoparticles (Au NPs)@g-C₃N₄ composite was synthesized by loading Au NPs on carbon nitride material, and it was immobilized on the surface of glassy carbon electrode by chitosan (Chit) as the substrate of electrochemical aptasensors. An ochratomycin A electrochemical aptasensor was constructed by hybridizing DNA1 and ochratoxin A (OTA) aptamers. The resulting hybrid strands were immobilized on the substrate of glassy carbon electrode. Electrochemical alternating current impedance (EIS) was used to detect the impedance value of the aptasensor when incubating different concentrations of OTA. The impedance value is inversely ratio to the concentration of OTA, achieving quantitative detection of OTA. The aptasensor can detect OTA with a linear range of 0.5-100 ng/mL, the linear correlation coefficient is 0.9506, and the detection limit is 0.167 ng/mL. This aptasensor provides a novel and efficient method for detecting OTA.
赭曲霉毒素对人类具有致畸性、致癌性和免疫毒性。它存在于许多食物中，因此检测食物中的赭曲霉毒素尤为重要。本文通过将金纳米粒子（Au NPs）负载在氮化碳材料上合成了金纳米粒子（Au NPs）@g-C₃N₄复合材料，并通过壳聚糖（Chit）将其固定在玻碳电极表面，作为电化学适体传感器的基底。通过杂交 DNA1 和赭曲霉毒素 A（OTA）适体构建了赭曲霉毒素 A 电化学适体传感器。所得杂交链被固定在玻碳电极的基底上。电化学交流阻抗（EIS）用于检测在孵育不同浓度 OTA 时适体传感器的阻抗值。阻抗值与 OTA 的浓度呈反比，实现了 OTA 的定量检测。该适体传感器可检测 OTA 的线性范围为 0.5-100ng/mL，线性相关系数为 0.9506，检测限为 0.167ng/mL。该适体传感器为检测 OTA 提供了一种新颖有效的方法。
