Texas Tech University, 2500 Broadway, Lubbock, Texas 79409, USA.
J Wildl Dis. 2020 Jul;56(3):702-706. doi: 10.7589/2019-08-212. Epub 2020 Apr 3.
Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. The amphibian pathogens () and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.
环境 DNA(eDNA)方法为病原体的检测提供了新的选择。在美国德克萨斯州，两栖动物病原体（）和蛙病毒的研究相对较少，因此我们应用 eDNA 检测方法对德克萨斯狭长地带附近上布拉佐斯河流域的这些病原体进行监测。我们从德克萨斯州拉伯克市及其周边的五个城市泻湖和一个水库中采集水样。定量 PCR 在一个泻湖中检测到了和蛙病毒，这是该地区首次同时检测到这两种病原体。基于这些结果，我们建议在整个地区加强对病原体和两栖动物疾病症状的监测。
