Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.
Curr Opin Chem Biol. 2020 Oct;58:1-9. doi: 10.1016/j.cbpa.2020.02.007. Epub 2020 Mar 31.
The review gives a large overview of the strategies used for protein synthesis by chemoselective peptide segment ligation on a solid support. It discusses also important aspects that remain to be explored to further develop the technology such as the role of the solid support on reactant diffusion rates, on ligation kinetics, as well as on the folding and functionality of the proteins attached to the solid support.
该综述概述了在固体载体上通过化学选择性肽段连接进行蛋白质合成的策略。它还讨论了一些有待进一步探索的重要方面，以进一步发展该技术，如固体载体对反应物扩散速率、连接动力学以及与固体载体连接的蛋白质折叠和功能的影响。
