辅助生殖技术受孕小鼠中 TH1、TH2、TH17 平衡的改变。

Altered TH1, TH2, TH17 balance in assisted reproductive technology conceived mice.

机构信息

Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran.

Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.

出版信息

J Reprod Immunol. 2020 Jun;139:103117. doi: 10.1016/j.jri.2020.103117. Epub 2020 Mar 16.

DOI:10.1016/j.jri.2020.103117

PMID:32244165

Abstract

AIM AND PURPOSE

The higher prevalence rate of different diseases may accentuate the possible alteration of the immune system in individuals conceived through the assisted reproductive technologies (ART). The present study was conducted to evaluate the TH1, TH2, TH17 balance in intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) - conceived mice in comparison to naturally conceived offspring.

METHODS

Mice (6-8 weeks) were divided into three groups (IVF- conceived, ICSI- conceived and naturally conceived). They were subjected to subcutaneous immunization witMycobacterium bovis Bacille Calmette-Guérin (BCG). The blood samples were taken and the sera were separated. Then the spleens were surgically removed at the time the mice were sacrificed. Serum levels of IFN-γ, IL-17A and IL-4 were detected by ELISA. Then the proportion of TCD4 cells possessing the T-bet+, GATA3+, and ROR-γt + were measured using FACS caliber flow cytometer.

RESULTS

In comparison with naturally conceived mice, intracellular expression of T-bet and serum levels of IFN-γ were significantly decreased in ART- conceived mice. Moreover, ART- conceived offspring showed marked increase in IL-4 and IL-17A.

CONCLUSION

It is concluded that compared to naturally conceived male mice, ART- conceived counterparts exhibit less efficient immune responses against BCG through further promotion of humoral and inflammatory related immune response characteristics.

摘要

目的和宗旨

不同疾病的高发率可能会加剧辅助生殖技术（ART）受孕个体免疫系统的可能改变。本研究旨在评估与自然受孕后代相比，卵胞浆内单精子注射（ICSI）和体外受精（IVF）受孕小鼠的 TH1、TH2、TH17 平衡。

方法

将小鼠（6-8 周）分为三组（IVF 受孕、ICSI 受孕和自然受孕）。它们接受牛分枝杆菌卡介苗（BCG）皮下免疫。采集血液样本并分离血清。然后在处死小鼠时通过手术切除脾脏。通过 ELISA 检测血清中 IFN-γ、IL-17A 和 IL-4 的水平。然后使用 FACS caliber 流式细胞仪测量具有 T-bet+、GATA3+和 ROR-γt+的 TCD4 细胞的比例。