Lin Hsiu-Chin, Wong Yue Him, Tsang Ling Ming, Chu Ka Hou, Qian Pei-Yuan, Chan Benny K K
a Biodiversity Research Center, Academia Sinica , Taipei 115 , Taiwan.
Biofouling. 2014 Feb;30(2):169-81. doi: 10.1080/08927014.2013.853051. Epub 2013 Dec 12.
This is the first study applying Next-Generation Sequencing (NGS) technology to survey the kinds, expression location, and pattern of adhesion-related genes in a membranous-based barnacle. A total of 77,528,326 and 59,244,468 raw sequence reads of total RNA were generated from the prosoma and the basis of Tetraclita japonica formosana, respectively. In addition, 55,441 and 67,774 genes were further assembled and analyzed. The combined sequence data from both body parts generates a total of 79,833 genes of which 47.7% were shared. Homologues of barnacle cement proteins - CP-19K, -52K, and -100K - were found and all were dominantly expressed at the basis where the cement gland complex is located. This is the main area where transcripts of cement proteins and other potential adhesion-related genes were detected. The absence of another common barnacle cement protein, CP-20K, in the adult transcriptome suggested a possible life-stage restricted gene function and/or a different mechanism in adhesion between membranous-based and calcareous-based barnacles.
这是第一项应用新一代测序（NGS）技术来调查基于膜的藤壶中黏附相关基因的种类、表达位置和模式的研究。分别从台湾笠藤壶的头胸部和基部产生了总共77,528,326条和59,244,468条总RNA的原始序列读数。此外，还进一步组装和分析了55,441个和67,774个基因。来自身体两个部位的合并序列数据总共产生了79,833个基因，其中47.7%是共享的。发现了藤壶黏附蛋白——CP-19K、-52K和-100K——的同源物，并且它们都在黏附腺复合体所在的基部大量表达。这是检测到黏附蛋白和其他潜在黏附相关基因转录本的主要区域。在成年转录组中未发现另一种常见的藤壶黏附蛋白CP-20K，这表明可能存在与生命阶段相关的基因功能限制和/或基于膜的藤壶和基于钙质的藤壶之间不同的黏附机制。
