Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, E08028 Barcelona, Spain.
Research Institute in Food Nutrition and Food Safety, University of Barcelona, E08921 Barcelona, Spain.
Molecules. 2020 Apr 1;25(7):1623. doi: 10.3390/molecules25071623.
Society's interest in the quality of food products with certain attributes has increased, the attribute of a Protected Designation of Origin (PDO) being an effective tool to guarantee the quality and geographical origin of a given food product. In Spain, two paprika production areas with PDO (La Vera and Murcia) are recognized. In the present work, targeted UHPLC-HRMS polyphenolic and capsaicinoid profiling through the TraceFinder screening software, using homemade accurate mass databases, was proposed as a source of chemical descriptors, to address the characterization, classification, and authentication of paprika. A total of 126 paprika samples from different production regions-Spain (La Vera PDO and Murcia PDO) and the Czech Republic, each including different flavor varieties, were analyzed. UHPLC-HRMS polyphenolic profiles showed to be good chemical descriptors to achieve paprika classification and authentication, based on the production region, through principal component analysis and partial least squares regression-discriminant analysis, with classification rates of 82%, 86%, and 100% for La Vera PDO, Murcia PDO, and the Czech Republic, respectively. In addition, a perfect classification was also accomplished among the flavor varieties for the Murcia PDO and Czech Republic samples. By employing the UHPLC-HRMS polyphenolic and capsaicinoid profiles as chemical descriptors, acceptable discrimination among La Vera PDO flavor varieties was also achieved.
社会对具有某些属性的食品质量的关注度有所提高，原产地保护名称 (PDO) 的属性是保证特定食品质量和地理来源的有效工具。在西班牙，有两个具有 PDO（拉韦拉和穆尔西亚）的辣椒粉生产区得到认可。在本工作中，通过 TraceFinder 筛选软件，使用自制的精确质量数据库，提出了靶向 UHPLC-HRMS 多酚和辣椒素特征分析方法，作为化学描述符的来源，以解决辣椒粉的表征、分类和鉴定问题。总共分析了来自不同生产地区的 126 个辣椒粉样本——西班牙（拉韦拉 PDO 和穆尔西亚 PDO）和捷克共和国，每个样本都包括不同的风味品种。UHPLC-HRMS 多酚谱被证明是实现基于生产地区的辣椒粉分类和鉴定的良好化学描述符，通过主成分分析和偏最小二乘回归判别分析，拉韦拉 PDO、穆尔西亚 PDO 和捷克共和国的分类率分别为 82%、86%和 100%。此外，还可以对穆尔西亚 PDO 和捷克共和国样本的风味品种进行完美分类。通过将 UHPLC-HRMS 多酚和辣椒素特征作为化学描述符，还可以在拉韦拉 PDO 风味品种之间实现可接受的区分。
