Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Kyonggi-do 13488, Korea.
Int J Mol Sci. 2020 Mar 20;21(6):2153. doi: 10.3390/ijms21062153.
Obesity results from an imbalance between energy intake and energy expenditure, in which excess fat is stored as triglycerides (TGs) in white adipocytes. Recent studies have explored the anti-obesity effects of certain edible phytochemicals, which suppress TG accumulation and stimulate a brown adipocyte-like phenotype in white adipocytes. Gomisin N (GN) is an important bioactive component of , a woody plant endemic to Asia. GN has antioxidant, anti-inflammatory and hepatoprotective effects in vivo and in vitro. However, the anti-obesity effects of GN in lipid metabolism and adipocyte browning have not yet been investigated. In the present study, we aimed to determine whether GN suppresses lipid accumulation and regulates energy metabolism, potentially via AMP-activated protein kinase (AMPK), in 3T3-L1 adipocytes. Our findings demonstrate that GN inhibited adipogenesis and lipogenesis in adipocyte differentiation. Also, GN not only increased the expression of thermogenic factors, including uncoupling protein 1 (UCP1), but also enhanced fatty acid oxidation (FAO) in 3T3-L1 cells. Therefore, GN may have a therapeutic benefit as a promising natural agent to combat obesity.
肥胖是由于能量摄入和能量消耗之间失衡导致的，多余的脂肪以三酰甘油（TGs）的形式储存在白色脂肪细胞中。最近的研究探索了某些可食用植物化学物质的抗肥胖作用，这些物质可以抑制 TG 的积累并刺激白色脂肪细胞中类似于棕色脂肪细胞的表型。戈米辛 N（GN）是亚洲特有的木本植物 的一种重要生物活性成分。GN 在体内和体外具有抗氧化、抗炎和保肝作用。然而，GN 在脂质代谢和脂肪细胞棕色化中的抗肥胖作用尚未得到研究。在本研究中，我们旨在确定 GN 是否通过 AMP 激活的蛋白激酶（AMPK）抑制 3T3-L1 脂肪细胞中的脂质积累和调节能量代谢，从而发挥其作用。我们的研究结果表明，GN 抑制了脂肪细胞分化过程中的脂肪生成和脂生成。此外，GN 不仅增加了包括解偶联蛋白 1（UCP1）在内的产热因子的表达，还增强了 3T3-L1 细胞中的脂肪酸氧化（FAO）。因此，GN 可能具有治疗益处，是一种有前途的天然抗肥胖药物。
