Lucey E C, Stone P J, Christensen T G, Breuer R, Snider G L
Pulmonary Section, Boston Veterans Administration Medical Center, MA 02130.
Exp Lung Res. 1988;14(5):671-86. doi: 10.3109/01902148809087836.
A study was made of the evolution of emphysema and airway injury induced in the lungs of male golden Syrian hamsters by a single intratracheal injection of 350 micrograms human neutrophil elastase (HNE). Saline control and HNE-treated groups of 8 animals were studied 1, 3, 6, 12, and 18 months posttreatment. HNE treatment caused a significant increase in all lung volumes and a significant decrease in maximum expiratory flows at all study times. The mean linear intercept (MLI) values of the left lung were significantly increased over control values. There was no progression with time in MLI values, lung volumes, or lung compliance. Secretory-cell metaplasia was present at 1 month and persisted throughout the study. The HNE-treated lungs showed clusters of ferric iron-containing macrophages in the terminal airspaces. The amount of iron in the lungs, determined morphometrically, was greatest at 1 month, was decreased by 6 months, and then did not change further to 18 months. At 18 months the amount of iron was still significantly above control amounts. We conclude that the airway and parenchymal lesions induced by HNE persist without progression for 18 months. Clearance of ferric iron, which was probably a result of the hemorrhage induced by HNE treatment, continued for 6 months with no evident subsequent clearance.
对雄性叙利亚金黄地鼠经气管内单次注射350微克人中性粒细胞弹性蛋白酶（HNE）后肺部诱发的肺气肿和气道损伤的演变进行了研究。对盐水对照组和HNE处理组的8只动物在处理后1、3、6、12和18个月进行了研究。HNE处理在所有研究时间均导致所有肺容积显著增加，最大呼气流量显著降低。左肺的平均线性截距（MLI）值比对照值显著增加。MLI值、肺容积或肺顺应性均未随时间进展。在1个月时出现分泌细胞化生，并在整个研究过程中持续存在。HNE处理的肺在终末气腔内可见含铁巨噬细胞簇。通过形态计量学测定，肺中铁含量在1个月时最高，6个月时降低，然后到18个月不再进一步变化。在18个月时，铁含量仍显著高于对照量。我们得出结论，HNE诱发的气道和实质病变持续18个月无进展。三价铁的清除可能是HNE处理引起的出血的结果，持续6个月，随后无明显清除。
