Colorado State University, Colorado, USA.
Genevia Technologies, Helsinki, Finland.
Sci Rep. 2020 Apr 3;10(1):5873. doi: 10.1038/s41598-020-62364-6.
Laboratory strains, cell lines, and other genetic materials change hands frequently in the life sciences. Despite evidence that such materials are subject to mix-ups, contamination, and accumulation of secondary mutations, verification of strains and samples is not an established part of many experimental workflows. With the plummeting cost of next generation technologies, it is conceivable that whole genome sequencing (WGS) could be applied to routine strain and sample verification in the future. To demonstrate the need for strain validation by WGS, we sequenced haploid yeast segregants derived from a popular commercial mutant collection and identified several unexpected mutations. We determined that available bioinformatics tools may be ill-suited for verification and highlight the importance of finishing reference genomes for commonly used laboratory strains.
实验室品系、细胞系和其他遗传材料在生命科学领域经常转手。尽管有证据表明这些材料容易发生混淆、污染和次要突变的积累，但对品系和样本的验证并不是许多实验工作流程的既定部分。随着下一代技术成本的急剧下降，可以想象全基因组测序（WGS）将来可能会应用于常规的品系和样本验证。为了证明通过 WGS 进行品系验证的必要性，我们对来自一个受欢迎的商业突变体库的单倍体酵母分离子进行了测序，并鉴定出了几个意想不到的突变。我们确定现有的生物信息学工具可能不适合验证，并强调完成常用实验室品系参考基因组的重要性。
