Chemistry and Biology Department, Federal Technological University of Paraná, Curitiba, PR, 81280-340, Brazil.
Environ Monit Assess. 2020 Apr 4;192(5):264. doi: 10.1007/s10661-020-8235-0.
Malathion is a highly toxic organophosphate insecticide, being one of the most widely used in the world and is generally used for insect control in food production. Thus, ecotoxicological studies have been used to verify its toxic effects on aquatic organisms, such as Daphnia magna and biomarkers, as the comet assay. The comet assay is a microgel electrophoresis method for the detection and quantification of DNA strand breaks in individual cells. Cells were obtained from Daphnia magna after disaggregation of newborn organisms, exposed at concentrations of 0.23 μg L and 0.47 μg L for 48 h. Malathion has shown to cause damage to DNA of the exposed organisms. It was also observed the need of further studies to standardize the comet assay technique for Daphnia magna, once methodologies used present several differences.
马拉硫磷是一种高毒性的有机磷杀虫剂，是世界上使用最广泛的杀虫剂之一，通常用于食品生产中的昆虫控制。因此，生态毒理学研究已经被用于验证其对水生生物的毒性影响，如大型溞和生物标志物，如彗星试验。彗星试验是一种用于检测和定量个体细胞中 DNA 链断裂的微凝胶电泳方法。细胞是从小型溞的新生个体中分离出来的，在 0.23μg/L 和 0.47μg/L 的浓度下暴露 48 小时。马拉硫磷已被证明会对暴露的生物的 DNA 造成损伤。同时还观察到需要进一步研究来标准化大型溞的彗星试验技术，因为所使用的方法存在许多差异。
