Weis-Fogh U S
Institute for Experimental Research in Surgery, University of Copenhagen, Panum Institute, Denmark.
Eur Surg Res. 1988;20(5-6):381-9. doi: 10.1159/000128789.
The two-component tissue adhesive system where the one component is a concentrated human fibrinogen solution and the other component is a thrombin solution containing Ca2+ is becoming increasingly important in surgery. In the commercially available tissue adhesive, the fibrinogen is separated from pooled plasma. The risk of transmitting foreign immunogens and viruses, always present when foreign biological materials are used, will be eliminated if the fibrinogen is separated from the patient's own blood. A method using ethanol precipitation is described for preparing a concentrated fibrinogen solution from plasma separated from small amounts of blood. The method is fast, the final product can be obtained within 30-60 min after collection of the blood. The recovery is compared with the recovery obtained by separating the fibrinogen with ammonium sulfate precipitation and with cryoprecipitation. The method using ethanol is by far the most profitable, and the product is evaluated by experimental liver surgery in pigs.
由一种成分是浓缩人纤维蛋白原溶液而另一种成分是含Ca2+的凝血酶溶液组成的双组分组织黏合剂系统在外科手术中变得越来越重要。在市售的组织黏合剂中，纤维蛋白原是从混合血浆中分离出来的。如果纤维蛋白原是从患者自身血液中分离出来的，那么使用外来生物材料时一直存在的传播外来免疫原和病毒的风险将被消除。描述了一种使用乙醇沉淀法从少量血液分离出的血浆中制备浓缩纤维蛋白原溶液的方法。该方法速度快，在采血后30 - 60分钟内即可获得最终产品。将该方法的回收率与通过硫酸铵沉淀和冷沉淀分离纤维蛋白原所获得的回收率进行了比较。使用乙醇的方法是目前最具效益的，并且该产品通过猪的实验性肝脏手术进行了评估。
