Silberstein L E, Williams L J, Hughlett M A, Magee D A, Weisman R A
Departments of Pathology, Hospital of the University of Pennsylvania, Philadelphia.
Transfusion. 1988 Jul-Aug;28(4):319-21. doi: 10.1046/j.1537-2995.1988.28488265257.x.
Successful middle ear surgery requires the availability of a safe, effective bonding material. Side effects caused by synthetic materials have led to the use of biologic adhesives; however, they carry the risk of transmission of infectious disease if they are prepared from pooled human blood. A procedure for the production of an autologous fibrinogen-based adhesive using polyethylene glycol to precipitate the fibrinogen-factor XIII component from plasma is described. This procedure requires 40 ml of whole blood and approximately 3 hours' preparation time, and it can be performed in any blood bank with the facilities for sterile techniques. This adhesive has been used successfully for otologic surgery in 12 patients, and further study of the use of this biologic adhesive for other microsurgical techniques should be undertaken.
成功的中耳手术需要一种安全、有效的粘结材料。合成材料引起的副作用导致了生物粘合剂的使用；然而，如果它们是由混合人血制备的，则存在传染病传播的风险。本文描述了一种使用聚乙二醇从血浆中沉淀纤维蛋白原 - XIII因子成分来生产自体纤维蛋白原基粘合剂的方法。该方法需要40毫升全血和约3小时的制备时间，并且可以在任何具备无菌技术设施的血库中进行。这种粘合剂已成功用于12例患者的耳科手术，应进一步研究这种生物粘合剂在其他显微外科技术中的应用。
