Eggink G, de Waard P, Huijberts G N
Agrotechnological Research Institute (ATO-DLO), Wageningen, The Netherlands.
Can J Microbiol. 1995;41 Suppl 1:14-21. doi: 10.1139/m95-163.
Poly(hydroxyalkanoates) (PHAs) were isolated from Pseudomonas aeruginosa 44T1 cultivated on euphorbia oil and castor oil. With the aid of 2-D proton NMR spectra and proton-detected multiple bond coherence NMR spectra the structures of the PHAs were determined. In addition to the usual PHA constituents (C6-C14 3-hydroxy fatty acids), PHAs formed from euphorbia oil contained delta 8,9-epoxy-3-hydroxy-5c-tetradecenoate, and probably delta 6,7-epoxy-3-hydroxydodecanoate and delta 4,5-epoxy-3-hydroxydecanoate. These novel constituents account for approximately 15% of the total amount of monomers and are clearly generated via beta-oxidation of vernolic acid (delta 12,13-epoxy-9c-octadecenoic acid), the main component of euphorbia oil. In PHAs formed from castor oil, 7% of the monomers found were derived from ricinoleic acid (12-hydroxy-9c-octadecenoic acid). The presence of 3,8-dihydroxy-5c-tetradecenoate was clearly demonstrated. Furthermore, NMR analysis strongly suggested the presence of 3,6-dihydroxydodecanoate, 6-hydroxy-3c-dodecenoate, and 4-hydroxydecanoate.
聚羟基脂肪酸酯（PHA）是从在大戟油和蓖麻油上培养的铜绿假单胞菌44T1中分离得到的。借助二维质子核磁共振谱和质子检测多键相干核磁共振谱确定了PHA的结构。除了常见的PHA成分（C6 - C14 3 - 羟基脂肪酸）外，由大戟油形成的PHA还含有δ8,9 - 环氧 - 3 - 羟基 - 5c - 十四碳烯酸酯，可能还有δ6,7 - 环氧 - 3 - 羟基十二烷酸酯和δ4,5 - 环氧 - 3 - 羟基癸酸酯。这些新成分约占单体总量的15%，显然是通过大戟油的主要成分巴豆酸（δ12,13 - 环氧 - 9c - 十八碳烯酸）的β - 氧化产生的。在由蓖麻油形成的PHA中，发现7%的单体来自蓖麻油酸（12 - 羟基 - 9c - 十八碳烯酸）。明确证实了3,8 - 二羟基 - 5c - 十四碳烯酸酯的存在。此外，核磁共振分析强烈表明存在3,6 - 二羟基十二烷酸酯、6 - 羟基 - 3c - 十二碳烯酸酯和4 - 羟基癸酸酯。
