Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, 91510-970, Porto Alegre, Brazil.
Ottawa Institute of Systems Biology and Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
J Proteomics. 2020 May 30;220:103764. doi: 10.1016/j.jprot.2020.103764. Epub 2020 Apr 1.
The variation in the bioavailability of oxygen constitutes the environmental conditions found by bacteria in their passage through the host gastro-intestinal tract. Given the importance of oxygen in the defense mechanism of bacteria, it is important to understand how bacteria respond to this stress at a metabolic level. The probiotic strain Enterococcus durans LAB18S was cultivated under aerobic and anaerobic conditions using prebiotic oligosaccharides as carbon source. The whole cell proteome and secretome were analyzed through label-free quantitative proteomics approach. The results showed that the LAB18S isolate when grown with fructo-oligosacchrides (FOS) showed a higher number of differentially expressed proteins compared to samples with galacto-oligosaccharides (GOS) or glucose. Clinically important enzymes for the treatment of cancer, L-asparaginase and arginine deiminase, were overexpressed when the isolate was cultured in FOS. In addition, the absence of oxygen induced the strain to produce proteins related to cell multiplication, cell wall integrity and resistance, and HO detoxification. This study showed that E. durans LAB18S growing on FOS was stimulated to produce clinically important biomolecules, including proteins that have been investigated as potential antineoplastic agents. Significance: The probiotic strain E. durans LAB18S produce clinically relevant enzymes for the treatment of cancer when cultivated in symbiosis with fructo-oligosacchrides (FOS). In addition, proteins associated with cellular multiplication, cell wall integrity and resistance, and HO detoxification were induced under anaerobic growth. These characteristics could be relevant to support maintenance of intestinal health.
氧生物利用度的变化构成了细菌在宿主胃肠道中通过时所遇到的环境条件。鉴于氧在细菌防御机制中的重要性，了解细菌如何在代谢水平上对此应激做出反应非常重要。益生菌菌株 Enterococcus durans LAB18S 使用益生元低聚糖作为碳源在有氧和无氧条件下进行培养。通过无标记定量蛋白质组学方法分析全细胞蛋白质组和分泌组。结果表明，与用半乳糖低聚糖（GOS）或葡萄糖培养的样品相比，用果低聚糖（FOS）培养的 LAB18S 分离株显示出更多差异表达的蛋白质。用于治疗癌症的临床重要酶，L-天冬酰胺酶和精氨酸脱亚氨酶，在分离株用 FOS 培养时被过度表达。此外，缺氧诱导该菌株产生与细胞增殖、细胞壁完整性和抗性以及 HO 解毒相关的蛋白质。这项研究表明，在 FOS 上生长的 E. durans LAB18S 被刺激产生临床重要的生物分子，包括已被研究为潜在抗肿瘤剂的蛋白质。意义：当与果低聚糖（FOS）共生培养时，益生菌菌株 E. durans LAB18S 产生用于治疗癌症的临床相关酶。此外，在厌氧生长下诱导与细胞增殖、细胞壁完整性和抗性以及 HO 解毒相关的蛋白质。这些特征可能与支持维持肠道健康有关。
