Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, 91510-970, Porto Alegre, Brazil.
School of Pharmaceutical Sciences, Ottawa Institute of Systems Biology and Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada.
Probiotics Antimicrob Proteins. 2024 Dec;16(6):1954-1965. doi: 10.1007/s12602-023-10136-9. Epub 2023 Aug 17.
The gut microbiome plays a critical role to all animals and humans health. Methods based on ex vivo cultures are time and cost-effective solutions for rapid evaluation of probiotic effects on microbiomes. In this study, we assessed whether the protein secretome from the potential probiotic Enterococcus durans LAB18S grown on fructoligosaccharides (FOS) and galactoligosaccharides (GOS) had specific effects on ex vivo cultured intestinal microbiome obtained from a healthy individual. Metaproteomics was used to evaluate changes in microbial communities of the human intestinal microbiome. Hierarchical clustering analysis revealed 654 differentially abundant proteins from the metaproteome samples, showing that gut microbial protein expression varied on the presence of different E. durans secretomes. Increased amount of Bacteroidetes phylum was observed in treatments with secretomes from E. durans cultures on FOS, GOS and albumin, resulting in a decrease of the Firmicutes to Bacteroidetes (F/B) ratio. The most functionally abundant bacterial taxa were Roseburia, Bacteroides, Alistipes and Faecalibacterium. The results suggest that the secretome of E. durans may have favorable effects on the intestinal microbial composition, stimulating growth and different protein expression of beneficial bacteria. These findings suggest that proteins secreted by E. durans growing on FOS and GOS have different effects on the modulation of gut microbiota functional activities during cultivation.
肠道微生物群对所有动物和人类的健康都起着至关重要的作用。基于体外培养的方法是快速评估益生菌对微生物组影响的经济有效的解决方案。在这项研究中，我们评估了潜在益生菌屎肠球菌 LAB18S 在果寡糖 (FOS) 和半乳糖寡糖 (GOS) 上生长时的蛋白质分泌组是否对来自健康个体的体外培养肠道微生物组有特定影响。代谢蛋白质组学用于评估人类肠道微生物组的微生物群落变化。层次聚类分析显示，来自代谢蛋白质组样品的 654 种差异丰富的蛋白质，表明肠道微生物蛋白质表达因不同的屎肠球菌分泌组的存在而不同。在 FOS、GOS 和白蛋白上培养的屎肠球菌分泌组处理中观察到厚壁菌门数量增加，导致厚壁菌门与拟杆菌门（F/B）的比例降低。功能上最丰富的细菌分类群是罗斯伯里氏菌、拟杆菌属、阿里斯派斯菌属和粪杆菌属。结果表明，屎肠球菌的分泌组可能对肠道微生物组成有有利影响，刺激有益细菌的生长和不同的蛋白质表达。这些发现表明，在培养过程中，在 FOS 和 GOS 上生长的屎肠球菌分泌的蛋白质对肠道微生物群功能活性的调节有不同的影响。
