Nouri Gharajalar Sahar, Hassanzadeh Parviz, Hosseinali Nejad Nasrin
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Comp Immunol Microbiol Infect Dis. 2020 Mar 29;71:101474. doi: 10.1016/j.cimid.2020.101474.
Campylobacter jejuni and coli are zoonotic bacteria commonly associated With human diarrhea and usually transmit through consumption of meat and poultry contaminated products such as heart and liver. Cytolethal distending toxin (cdt) in Campylobacter spp. is among the significant virulence factors of these bacteria in the intestine. The purpose of this study was to determine the prevalence of Campylobacter spp. and presence of cdt genes among isolated bacteria.
In this cross sectional study, 100 chicken livers were examined. Detection, bacterial enumeration and isolation of Campylobacter spp. was done using Campylobacter selective agar media containing Campylobacter growth supplement, gram staining, catalase and oxidase production, hippurate hydrolysis and PCR molecular technique. Also the presence of cdt genes were detected using PCR assay.
From 100 studied liver samples, 43 were contaminated with Campylobacter spp. Among them 31(72 %) samples had Campylobacter jejuni and 12 (28 %) had Campylobacter coli. All Campylobacter jejuni isolates contained cdtA ،cdtB and cdtC genes. However, all of these genes detected in 9 (75 %) of isolated coli.
The results of this study showed that great percentages of chicken livers in Tabriz were contaminated with Campylobacter.
空肠弯曲菌和结肠弯曲菌是常见的人畜共患病原菌，通常与人类腹泻相关，且常通过食用受污染的肉类和禽类产品（如心脏和肝脏）传播。弯曲菌属中的细胞致死膨胀毒素（cdt）是这些细菌在肠道中的重要毒力因子之一。本研究的目的是确定弯曲菌属的流行情况以及分离出的细菌中cdt基因的存在情况。
在这项横断面研究中，检查了100个鸡肝。使用含有弯曲菌生长补充剂的弯曲菌选择性琼脂培养基进行弯曲菌属的检测、细菌计数和分离，进行革兰氏染色、过氧化氢酶和氧化酶产生试验、马尿酸盐水解试验以及PCR分子技术。此外，使用PCR检测法检测cdt基因的存在情况。
在100个研究的肝脏样本中，43个被弯曲菌属污染。其中31个（72%）样本为空肠弯曲菌，12个（28%）为结肠弯曲菌。所有空肠弯曲菌分离株均含有cdtA、cdtB和cdtC基因。然而，在9个（75%）分离出的结肠弯曲菌中检测到了所有这些基因。
本研究结果表明，大不里士地区很大比例的鸡肝被弯曲菌污染。
