Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2020 Mar;168(5):704-708. doi: 10.1007/s10517-020-04784-y. Epub 2020 Apr 4.
We performed a morphometric analysis of mesenteric lymph nodes in rats with breast cancer induced by administration of N-methyl-N-nitrosourea. The volume of the paracortical zone and the number of mature plasma cells in the medullary sinuses were increased and the volume of lymphoid nodules with germinal centers and the number of macrophages were decreased in the group with tumor resection and chemotherapy in comparison with untreated rats with breast cancer. In rats receiving fragmented human double-stranded DNA in combination with adjuvant therapy, the volume of marginal and medullary sinuses and the number of small lymphocytes and macrophages in the paracortical zone increased in comparison with the group receiving chemotherapy without DNA preparation; the volume of lymphoid nodules with germinal centers returned to the level observed in the intact group; the volume of medullary substance and proliferative activity of cells in the germinal centers and medullary substance decreased, the number of mature plasma cells returned to normal in the medullary substance and decreased in the medullary sinuses.
我们对 N-甲基-N-亚硝脲诱导的乳腺癌大鼠肠系膜淋巴结进行了形态计量学分析。与未经治疗的乳腺癌大鼠相比，肿瘤切除和化疗组大鼠的皮质旁区体积增大，髓窦内成熟浆细胞数量增加，生发中心淋巴小结和巨噬细胞数量减少。在联合佐剂治疗接受人源性双链 DNA 片段的大鼠中，与未接受 DNA 制备的化疗组相比，边缘窦和髓窦体积以及皮质旁区小淋巴细胞和巨噬细胞数量增加；生发中心淋巴小结体积恢复到完整组观察到的水平；髓质物质的体积和生发中心及髓质物质的细胞增殖活性降低，髓质物质中的成熟浆细胞数量恢复正常，髓窦中的成熟浆细胞数量减少。
