Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2020 Mar;168(5):681-687. doi: 10.1007/s10517-020-04780-2. Epub 2020 Apr 4.
We studied the levels of serum and lymph cytokines involved in the pathogenesis of BC. BC was induced by injection of N-methyl-N-nitrosourea to Wistar rats. The animals underwent surgery, or received polychemotherapy (cyclophosphamide, methotrexate, and 5-fluorouracil), or surgical treatment was combined with polychemotherapy; in a special group, Panagen (fragmented DNA) was added to polychemotherapy. Cytokine concentration in the lymph was measured using Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad). In rats with BC, the content of most studied cytokines (IL-1β, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1α, MIP-3α, RANTES, TNFα, and MCP-1) in the lymph and blood was significantly higher, while the content of IL-10 and GRO/KC was lower than in intact animals. Surgical resection of the tumor led to a significant decrease in the content of both pro- and anti-inflammatory cytokines in the lymph. Polychemotherapy led to a significant decrease in the content of IL-1β, IL-4, IL-6, IL-7, MIP-1α, MIP-3α, and RANTES in the serum and lymph. Comparison of the cytokine content in the serum and lymph of operated animals after polychemotherapy with and without Panagen showed that the content of most cytokines (IL-5, IL-6, IL-7, IL-10, IL-13, IL-17A , IL-18, GRO/KC, IFNγ, and MIP-3α) was higher after Panagen administration.
我们研究了参与 BC 发病机制的血清和淋巴细胞因子水平。BC 通过向 Wistar 大鼠注射 N-甲基-N-亚硝脲诱导。动物接受手术、多化疗（环磷酰胺、甲氨蝶呤和 5-氟尿嘧啶）或手术治疗联合多化疗；在一个特殊组中，向多化疗中添加 Panagen（片段化 DNA）。使用 Bio-Plex Pro Rat Cytokoness 24-Plex Assay 测试系统（Bio-Rad）测量淋巴中的细胞因子浓度。在患有 BC 的大鼠中，大多数研究细胞因子（IL-1β、IL-2、IL-4、IL-6、IL-7、IL-12、IL-13、MIP-1α、MIP-3α、RANTES、TNFα 和 MCP-1）的含量在血液和淋巴中均显着升高，而 IL-10 和 GRO/KC 的含量则低于完整动物。肿瘤的手术切除导致淋巴中促炎和抗炎细胞因子的含量显着降低。多化疗导致血清和淋巴中 IL-1β、IL-4、IL-6、IL-7、MIP-1α、MIP-3α 和 RANTES 的含量显着降低。比较多化疗后加用和不加用 Panagen 的手术动物血清和淋巴中细胞因子含量，发现大多数细胞因子（IL-5、IL-6、IL-7、IL-10、IL-13、IL-17A、IL-18、GRO/KC、IFNγ 和 MIP-3α）的含量在 Panagen 给药后更高。
