School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, China.
Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212018, Jiangsu, China.
Virol J. 2020 Apr 6;17(1):48. doi: 10.1186/s12985-020-01321-1.
Cypoviruses (CPVs) are RNA viruses with segmented double-stranded genome and major pathogens of various insects, including economic insects like silkworms and pest insects for agricultural crops and forests. Genome reassortment and recombination are common phenomenon for viruses as a mechanism to expand host range and increase virulence. In the present study, we analyzed the reassortant and recombination events for CPVs. The results showed that two genome segments (S1 and S4) of BmCPV1-YN shared higher nucleotide identity with the corresponding segment of BmCPV1-I while others were all more closely to BmCPV1-SZ, suggesting BmCPV1-YN was originated from reassortant events between BmCPV1-I and BmCPV1-SZ. Recombination analyses revealed that S6 of BmCPV1-YN was a recombinant segment derived from BmCPV1-I and BmCPV1-SZ, and S10 of DpCPV1 was a recombinant segment emerged from BmCPV1-I and LdCPV1. Our findings provide the evidence for the fact that CPVs could undergo reassortant and recombinant events and enrich the knowledge about etiology and molecular epidemiology of CPVs.
Cypoviruses (CPVs) 是具有分段双链 RNA 基因组的 RNA 病毒，是包括家蚕和农业作物及森林害虫在内的各种昆虫的主要病原体。基因组重排和重组是病毒的常见现象，是扩大宿主范围和增加毒力的一种机制。在本研究中，我们分析了 CPVs 的重排和重组事件。结果表明，BmCPV1-YN 的两个基因组片段（S1 和 S4）与 BmCPV1-I 的相应片段具有更高的核苷酸同一性，而其他片段均与 BmCPV1-SZ 更为接近，表明 BmCPV1-YN 是由 BmCPV1-I 和 BmCPV1-SZ 之间的重排事件产生的。重组分析表明，BmCPV1-YN 的 S6 是源自 BmCPV1-I 和 BmCPV1-SZ 的重组片段，DpCPV1 的 S10 是源自 BmCPV1-I 和 LdCPV1 的重组片段。我们的研究结果为 CPVs 可以发生重排和重组事件提供了证据，丰富了 CPVs 的病因学和分子流行病学知识。
