Laboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.
Institute of Biology, Irkutsk State University, Irkutsk, Russia.
Microbiol Spectr. 2023 Jun 15;11(3):e0385522. doi: 10.1128/spectrum.03855-22. Epub 2023 May 8.
Now more than ever researchers provide more and more evidence that it is necessary to develop an ecologically friendly approach to pest control. This is reflected in a sharp increase in the value of the biological insecticide market in recent decades. In our study, we found a virus strain belonging to the genus (Reoviridae); the strain was isolated from , possessing attractive features as a candidate for mass production of biological agents for lepidopteran-pest control. We describe the morphological, molecular, and ecological features of the new strain. This strain was found to be highly virulent to (the half-lethal dose is 25 occlusion bodies per second-instar larva) and to have a relatively wide host range (infecting representatives of five families of Lepidoptera: Erebidae, Sphingidae, Pieridae, Noctuidae, and Lasiocampidae). The virus strain showed a strong interaction with a nontoxic adjuvant (optical brightener), which decreased the lethal dose for both main and alternative hosts, decreased lethal time, and may expand the host range. Moreover, we demonstrated that the insecticidal features were preserved after passaging through the most economically suitable host. By providing strong arguments for the possible use of this strain in pest control, we call on virologists, pest control specialists, and molecular biologists to give more attention to the genus, which may lead to new insights in the field of pest control research and may provide significant advantages to compare with baculoviruses and Bacillus thuringiensis products which are nowadays main source of bioinsecticides. In this article, we describe a newly discovered cypovirus strain that displays features ideally suited for the development of a modern biological insecticide: high potency, relatively broad host range, true regulating effect, flexible production (possibility to choose host species for production), interaction with enhancing adjuvants, and ecologically friendly. Based on an alignment of CPV genomes, we suggest that the enhanced host range of this new strain is the sequence of evolutionary events that occurred after coinfections involving different CPV species within the same host. These findings suggest that we need to positively reconsider CPVs as prospective agents as biocontrol products.
现在，越来越多的研究人员提供了越来越多的证据，证明有必要开发一种对生态友好的害虫控制方法。这反映在最近几十年生物杀虫剂市场价值的急剧增长。在我们的研究中，我们发现了一种属于（呼肠孤病毒科）属的病毒株；该菌株是从分离出来的，具有作为鳞翅目害虫生物防治剂大规模生产的候选物的有吸引力的特征。我们描述了新菌株的形态、分子和生态特征。该菌株对（半数致死剂量为每龄幼虫 25 个包埋体）表现出高度的毒力，并且具有相对较宽的宿主范围（感染鳞翅目五个科的代表：夜蛾科、天蛾科、凤蝶科、夜蛾科和枯叶蛾科）。该病毒株与一种无毒佐剂（光学增白剂）表现出强烈的相互作用，降低了主要和替代宿主的致死剂量，缩短了致死时间，并可能扩大宿主范围。此外，我们证明，在通过最经济适用的宿主传代后，杀虫特征得以保留。通过为该菌株在害虫防治中的可能应用提供有力论据，我们呼吁病毒学家、害虫防治专家和分子生物学家更多地关注该属，这可能为害虫防治研究领域带来新的见解，并可能与当今生物杀虫剂的主要来源杆状病毒和苏云金芽孢杆菌产品相比具有显著优势。在本文中，我们描述了一种新发现的 cypovirus 菌株，该菌株具有非常适合开发现代生物杀虫剂的特征：高效力、相对较宽的宿主范围、真正的调节作用、灵活的生产（可选择生产宿主物种的可能性）、与增强佐剂的相互作用以及生态友好性。基于 CPV 基因组的比对，我们建议该新菌株的增强宿主范围是在同一宿主内不同 CPV 物种共同感染后发生的进化事件序列。这些发现表明，我们需要积极重新考虑 CPV 作为有前途的生物防治产品的代理。
