Laboratory of Advanced Materials Chemistry, Advanced Institute of Materials Science, Ton Duc Thang University, Ho Chi Minh City, Viet Nam; Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Viet Nam.
College of Pharmacy, Yeungnam University, Gyeongbuk 38541, Republic of Korea.
Bioorg Med Chem Lett. 2020 Jun 1;30(11):127145. doi: 10.1016/j.bmcl.2020.127145. Epub 2020 Mar 29.
Two new chlorinated secondary metabolites, saccharochlorines A and B (1 and 2), were isolated from the saline cultivation of a marine-derived bacterium Saccharomonospora sp. (KCTC-19160). The chemical structures of the saccharochlorines were elucidated by 2D NMR and MS spectroscopic data. Saccharochlorines A and B (1 and 2) exhibit weak inhibition of β-secretase (BACE1) in biochemical inhibitory assay, but they induced the release of Aβ (1-40) and Aβ (1-42) in H4-APP neuroglial cells. This discrepancy might be derived from the differences between the cellular and sub-cellular environments or the epigenetic stimulation of BACE1 expression.
两种新的氯化次生代谢产物，saccharochlorines A 和 B（1 和 2），从海洋来源的细菌 Saccharomonospora sp.（KCTC-19160）的盐培养物中分离出来。通过 2D NMR 和 MS 光谱数据阐明了 saccharochlorines 的化学结构。saccharochlorines A 和 B（1 和 2）在生化抑制测定中表现出对β-分泌酶（BACE1）的弱抑制作用，但它们在 H4-APP 神经胶质细胞中诱导 Aβ（1-40）和 Aβ（1-42）的释放。这种差异可能源于细胞和亚细胞环境之间的差异，或者是 BACE1 表达的表观遗传刺激。
