Unidad Mixta Gestión Biotecnológica de Plagas UV-IVIA, Department of Genetics, Universitat de València, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina (ERI-BIOTECMED), Burjassot, Valencia, Spain.
Unidad Mixta Gestión Biotecnológica de Plagas UV-IVIA, Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Valencia, Spain.
Insect Mol Biol. 2020 Aug;29(4):373-380. doi: 10.1111/imb.12642. Epub 2020 Apr 20.
The implementation of Integrated Pest Management in current agricultural practice is a convenient and very effective strategy to keep pest populations under control. The use of biological control agents, such as Phytoseiulus persimilis, is key for the success of such an approach. This predatory mite is widely used as it is very effective for controlling Tetranychus urticae, one of the most devastating crop pests. Here, we identify several mutations located in the voltage-gated sodium channel (VGSC) of commercially sourced P. persimilis that correlate with a reduced susceptibility to the pyrethroid deltamethrin. We found that the mites sourced from two different biocontrol product companies have intrinsic genotypic differences that correlate with their phenotype when tested with different concentrations of deltamethrin. Mites from Syngenta Bioline, carrying the mutations M918L and A1536T, were able to survive deltamethrin concentrations of up to 10 ppm, while the mites from Koppert Biological Systems, with the combination M918L, L925V and S1539T, survived treatment with 40 ppm. All of the point mutations identified in the predatory mite samples are located in a particular region of the VGSC, previously proposed as the binding site for this family of pesticides and identified as a 'hot spot' for resistance.
在当前农业实践中实施综合虫害管理是一种控制害虫种群的便捷且非常有效的策略。利用生物防治剂，如智利小植绥螨，是这种方法成功的关键。这种捕食螨因其对棉叶螨的高效控制而被广泛应用，棉叶螨是最具破坏性的作物害虫之一。在这里，我们鉴定了几种位于商业来源的智利小植绥螨电压门控钠离子通道（VGSC）中的突变，这些突变与对拟除虫菊酯溴氰菊酯的敏感性降低有关。我们发现，来自两家不同生物防治产品公司的螨虫具有内在的基因型差异，这些差异与它们在用不同浓度溴氰菊酯测试时的表型相关。携带 M918L 和 A1536T 突变的 Syngenta Bioline 螨虫能够在高达 10ppm 的溴氰菊酯浓度下存活，而带有 M918L、L925V 和 S1539T 组合的 Koppert Biological Systems 螨虫则能够在 40ppm 的处理下存活。在捕食性螨虫样本中鉴定出的所有点突变都位于 VGSC 的一个特定区域，该区域先前被提议为该类杀虫剂的结合位点，并被确定为抗性的“热点”。
