Efficacy of ajwain (Trachyspermum ammi L.) seed at graded levels of dietary threonine on growth performance, serum metabolites, intestinal morphology and microbial population in broiler chickens.

The present study investigated the influence of dietary threonine (Thr) levels and ajwain seed (AS) on the growth performance, immunity, blood metabolites, ileal microflora and jejunum morphology of broiler chickens. A total of 600-day-old male broiler chickens (Ross 308) were randomly allocated among five replicates of eight dietary treatments according to a 4 × 2 factorial arrangement of treatments, including four different levels of Thr (100, 105, 110 and 115% of requirements) and two inclusion rates of AS (0 or 10 g/kg of the diet). Body weight gain and FCR improved in broiler chickens fed 105% Thr supplement during the starter, finisher and whole periods of the experiment (p < .05). The broiler chickens fed AS supplemented diet had a greater weight gain (25-42 days) and better FCR (1-10 and 25-42 days) than those without AS (p < .05). A significant interaction was observed between Thr and AS supplementation for lymphocyte, heterophil/lymphocyte (H/L), bursa and spleen (p < .05). The liver enzyme activity of aspartate aminotransferase (AST) was higher in the chicks that received diet without AS supplement than those fed AS diet (p < .05). Inclusion of 105% dietary Thr and 10 g/kg AS increased the viable cell counts of Lactobacilli and decreased the population of the Escherichia coli in broilers (p < .05). In jejunum morphology, the broiler chickens fed 105% Thr diet had a greater villus length, width and crypt depth (p < .05). Also, the villus length was longer in broilers that received AS supplemented diet (p < .05). It is concluded that the dietary 105% of Thr and AS supplement individually improved growth performance, ileal microbial population and jejunum morphology of broiler chickens.