Rajasekariah G R, Puri P M, Chandrashekar R, Subrahmanyam D
Research Centre, Pharma Division, Hindustan Ciba-Geigy Ltd, Goregaon, Bombay, India.
Immunol Cell Biol. 1988 Oct-Dec;66 ( Pt 5-6):331-6. doi: 10.1038/icb.1988.43.
Active Brugia pahangi microfilariae (Mf) were injected into naive male BALB/c mice intraperitoneally. Microfilaraemia was studied for 28 days; and Mf circulated in blood in optimum numbers from 3 to 14 days. Anti-Mf response was assessed by the rate of disappearance of Mf from blood as well as their absence from visceral organs. Sonicated antigens of Mf (MfE) and whole worm extract of adults (WWE) induced absolute protection against the establishment of Mf in mice. This potent anti-Mf response elicited by sonicated antigens was intense, rapid and withstood repeated challenge infection. In comparison, immunization with in vitro excretory, secretory and metabolic antigens of Mf (MfESM) produced a partial but significant level of protection. Sera collected periodically from immunized animals showed antibody by micro-ELISA compared to sham-vaccinated controls. When Mf were used as targets along with the peritoneal exudate cells in vitro, sera from mice immunized with MfE and MfESM showed about four-fold cellular adherence to (Mf-ADCA) and 10-fold killing (Mf-ADCC) of Mf which was antibody-dependent. Some degree of correlation was apparent when the antibody levels, Mf-ADCA/Mf-ADCC activity, and Mf clearance were compared. This murine microfilaraemia model was therefore shown to be suitable for studying the host-protective immune response against filarial parasites.
将活性班氏吴策线虫微丝蚴（Mf）经腹腔注射入未感染的雄性BALB/c小鼠体内。对微丝蚴血症进行了28天的研究；微丝蚴在血液中以最佳数量循环的时间为3至14天。通过微丝蚴从血液中消失的速率以及在内脏器官中不存在微丝蚴来评估抗微丝蚴反应。微丝蚴的超声破碎抗原（MfE）和成虫的全虫提取物（WWE）可诱导小鼠对微丝蚴的建立产生绝对保护。超声破碎抗原引发的这种有效的抗微丝蚴反应强烈、迅速且能抵抗重复的攻击感染。相比之下，用微丝蚴的体外排泄、分泌和代谢抗原（MfESM）进行免疫产生了部分但显著水平的保护作用。与假疫苗接种对照组相比，定期从免疫动物采集的血清通过微量ELISA显示有抗体。当在体外将微丝蚴与腹腔渗出细胞一起作为靶标时，用MfE和MfESM免疫的小鼠血清显示出约四倍的细胞对微丝蚴的黏附（Mf-ADCA）和十倍的微丝蚴杀伤（Mf-ADCC），这是抗体依赖性的。当比较抗体水平、Mf-ADCA/Mf-ADCC活性和微丝蚴清除率时，明显存在一定程度的相关性。因此，该小鼠微丝蚴血症模型被证明适用于研究宿主针对丝虫寄生虫的保护性免疫反应。
