MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
School of Medicine, Hangzhou Normal University, Hangzhou 311121, China.
Anal Chem. 2020 May 5;92(9):6358-6365. doi: 10.1021/acs.analchem.9b05342. Epub 2020 Apr 16.
The novel 3D microfluidic concept of "lab-on-hollow fiber membrane (HFM)" was presented for multifunctional and rapid biological assays, integrating sample size sieving and colorimetric quantification in an HFM. Herein, microporous HFMs with a gradient pore size and high hydrophilic flux were used as microfluidic device substrates. The membrane pores selectively trapped macromolecules within the inner surface, while allowing free diffusion of smaller molecules, including glucose and protein. The microfluidic flow rate in HFM closely followed the Lucas-Washburn and Laplace's models, indicating that the microfluidics facilitated the upward flow of the fluid by microcapillary action without external pumping. Subsequently, for sensing of different biomolecules, a highly sensitive fluorescent or optical chromogenic reagent was immobilized in HFM by an electrostatic interaction. Pyronin G fluorescence reagent was quenched by blood glucose, and the quenching efficiency showed a good linear correlation with glucose concentration (1-22 mM, = 0.997). Moreover, this sensing platform was then further applied for the analysis of urine protein or glucose in the visible spectrum, with a wide testing range. Compared to traditional 2D flat membrane devices, this 3D-HFM microfluidic device exhibited excellent sensing versatility and color rendering uniformity with enhanced sensitivity. Target molecules screening, conditioning, enzymatic recognition, and signal readout of biomolecules have all been implemented on this device, which has paved the way to highly sensitive assays on point-of-care testing (POCT).
提出了一种新颖的 3D 中空纤维膜（HFM）微流控概念，用于多功能和快速生物分析，将样品尺寸筛分和 HFM 中的比色定量集成在一起。本文中，使用具有梯度孔径和高亲水性通量的微孔 HFMs 作为微流控器件基底。膜孔选择性地在内表面捕获大分子，同时允许较小分子（包括葡萄糖和蛋白质）自由扩散。HFM 中的微流体流速紧密遵循 Lucas-Washburn 和 Laplace 模型，表明微流控通过毛细作用促进了流体的向上流动，而无需外部泵送。随后，为了感测不同的生物分子，通过静电相互作用将高灵敏度的荧光或光学显色试剂固定在 HFM 中。吡咯红 G 荧光试剂被葡萄糖猝灭，猝灭效率与葡萄糖浓度（1-22mM， = 0.997）呈良好的线性关系。此外，该传感平台还进一步应用于可见光范围内尿液蛋白或葡萄糖的分析，具有较宽的测试范围。与传统的 2D 平面膜器件相比，这种 3D-HFM 微流控器件具有出色的传感多功能性和显色均匀性，灵敏度得到增强。已经在该器件上实现了对生物分子的靶分子筛选、调理、酶识别和信号读出，为即时检测（POCT）上的高灵敏度分析铺平了道路。
