Department of Microbiology, Faculty of Medicine, Kafkas University, Kars, Turkey.
Department of Virology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
Future Microbiol. 2020 Apr;15:401-412. doi: 10.2217/fmb-2020-0005. Epub 2020 Apr 6.
The objective of this study was to evaluate the possible protective effects of probiotic bacteria, especially and strains, on 4,4'-dichlorodiphenyltrichloroethane (DDT)-induced toxicity. For this reason, we evaluated the relationship between probiotics and toxicity by checking immunological and immunohistochemical parameters. Probiotic pretreatment was applied to 36 Wistar albino rats for 12 consecutive days. Serum aspartate aminotransferase and alanine aminotransferase levels were detected. CD3 and NF-κB staining methods were then performed by immunohistochemistry. Finally, pro- and anti-inflammatory cytokines were measured by ELISA. DDT caused a serious increase/decrease in some cytokine parameters. The effective dose was 1 × 10 colony-forming unit probiotic treatment. CD3 and NF-κB positivity were intense in DDT group whereas the intensity was reduced in probiotic treatment groups. The probiotic mixture has a potential to prevent inflammatory and oxidative stress related organ injuries. Further studies should be performed to explain the possible mechanisms.
本研究旨在评估益生菌，特别是 和 菌株对 4,4'-二氯二苯三氯乙烷（DDT）诱导的毒性的可能保护作用。为此，我们通过检查免疫和免疫组织化学参数来评估益生菌与毒性之间的关系。将益生菌预处理应用于 36 只 Wistar 白化大鼠连续 12 天。检测血清天冬氨酸氨基转移酶和丙氨酸氨基转移酶水平。然后通过免疫组织化学方法进行 CD3 和 NF-κB 染色。最后，通过 ELISA 测量促炎和抗炎细胞因子。DDT 导致某些细胞因子参数严重增加/减少。有效剂量为 1×10 菌落形成单位益生菌治疗。DDT 组 CD3 和 NF-κB 阳性较强，而益生菌治疗组阳性强度降低。益生菌混合物具有预防与炎症和氧化应激相关的器官损伤的潜力。应进行进一步的研究以解释可能的机制。
