Cheng Robert Y S, Patel Nimit L, Back Timothy, Basudhar Debashree, Somasundaram Veena, Kalen Joseph D, Wink David A, Ridnour Lisa A
Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick.
Small Animal Imaging Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc.
J Vis Exp. 2020 Mar 20(157). doi: 10.3791/60316.
Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less aggressive breast tumors, the 5-year survival rate of TNBC patients is 77% due to their characteristic drug-resistant phenotype and metastatic burden. Toward this end, murine models have been established aimed at identifying novel therapeutic strategies limiting TNBC tumor growth and metastatic spread. This work describes a practical guide for the TNBC orthotopic model where MDA-MB-231 breast cancer cells suspended in a basement membrane matrix are implanted in the fourth mammary fat pad, which closely mimics the cancer cell behavior in humans. Measurement of tumors by caliper, lung metastasis assessment via in vivo and ex vivo imaging, and molecular detection are discussed. This model provides an excellent platform to study therapeutic efficacy and is especially suitable for the study of the interaction between the primary tumor and distal metastatic sites.
三阴性乳腺癌（TNBC）是一种侵袭性乳腺癌亚型，治疗选择有限。与侵袭性较低的乳腺肿瘤患者相比，TNBC患者的5年生存率为77%，这是由于其具有耐药表型和转移负担的特征。为此，已建立小鼠模型，旨在确定限制TNBC肿瘤生长和转移扩散的新治疗策略。这项工作描述了TNBC原位模型的实用指南，即将悬浮在基底膜基质中的MDA-MB-231乳腺癌细胞植入第四乳腺脂肪垫，该模型密切模拟人类癌细胞行为。讨论了通过卡尺测量肿瘤、通过体内和体外成像评估肺转移以及分子检测。该模型为研究治疗效果提供了一个极好的平台，特别适合研究原发性肿瘤与远处转移部位之间的相互作用。
