Department of Otorhinolaryngology-Head and Neck Surgery, Queen Elizabeth Hospital and the University of Adelaide, Adelaide, SA, Australia.
School of Biology, Faculty of Science and Engineering, Flinders University of South Australia, Adelaide, SA, Australia.
Int Forum Allergy Rhinol. 2020 Oct;10(10):1141-1148. doi: 10.1002/alr.22575. Epub 2020 Jun 7.
Nasal topical treatments can provide an effective method of disease control for patients suffering from chronic rhinosinusitis (CRS). However, some frequently used formulations lack adequate evaluation on their safety. This study investigated the effect of 0.5% povidone-iodine (Nasodine) on the sinonasal epithelial barrier and ciliated human nasal epithelial cells (HNECs) in vitro.
Nasodine was applied to air-liquid interface (ALI) cultures of primary HNECs from CRS patients. Epithelial barrier structure was assessed by measuring the transepithelial electrical resistance (TEER), paracellular permeability, and immunolocalization of the zona occludens-1 (ZO-1) tight junction protein. Toxicity and ciliary beat frequency (CBF) were also studied.
Nasodine was not toxic and did not have detrimental effects on the paracellular permeability or CBF. Nasodine did not show a significant reduction in TEER with a 5-minute exposure; however, with a 30-minute exposure there was a significant reduction in TEER at 1 hour and at 4 hours after exposure.
Application of Nasodine to HNEC-ALI cultures in vitro for up to 30 minutes was not toxic and did not affect the paracellular permeability or CBF.
鼻腔局部治疗可为慢性鼻-鼻窦炎（CRS）患者提供有效的疾病控制方法。然而，一些常用的制剂在安全性方面缺乏足够的评估。本研究旨在体外研究 0.5%聚维酮碘（Nasodine）对鼻黏膜上皮屏障和人鼻黏膜纤毛上皮细胞（HNEC）的影响。
将 Nasodine 应用于 CRS 患者原代 HNEC 的气液界面（ALI）培养物。通过测量跨上皮电阻（TEER）、旁通透性以及紧密连接蛋白闭合蛋白-1（ZO-1）的免疫定位来评估上皮屏障结构。还研究了毒性和纤毛摆动频率（CBF）。
Nasodine 无毒性，对旁通透性或 CBF 没有不利影响。Nasodine 暴露 5 分钟时 TEER 无明显降低，但暴露 30 分钟时，暴露后 1 小时和 4 小时 TEER 明显降低。
将 Nasodine 应用于体外 HNEC-ALI 培养物中不超过 30 分钟是安全的，不会影响旁通透性或 CBF。
