Department of Otorhinolaryngology, Head and Neck Surgery, Medical Faculty OWL, Bielefeld University, Campus Klinikum Bielefeld, Teutoburger Str. 50, 33604, Bielefeld, Germany.
Labor Krone, Laboratory for Medical Diagnostics, Bad Salzuflen, Germany.
Eur Arch Otorhinolaryngol. 2021 Dec;278(12):5059-5067. doi: 10.1007/s00405-021-06873-8. Epub 2021 May 22.
The scope of this research endeavor was the determination of the applicability of over the counter mouthwash solutions in reducing the viral load in the saliva of COVID-19 patients and hence decreasing their infectivity. Beyond that, new experimental mouthwashes were investigated in terms of a possible positive immune modulation, which might offer an additional opportunity for a positive pharmaceutical effect.
The effectivity of the mouth washing solution was determined on 34 hospitalized COVID-19 patients by measuring the viral load by RT-qPCR in pharyngeal swabs, which were taken before and after rinsing. The inflammatory modulation thru the experimental solutions was assayed in an in vitro model of virus infected nasopharyngeal epithelium cells.
The clinical pilot study demonstrated that the mouth rinsing solution was able to reduce the viral load by about 90% in the saliva of most patients. This reduction was determined to persist for about 6 h. In the experimental solutions, the ingredients dexpanthenol and zinc were able to reduce the expression of proinflammatory cytokines in the cell culture model, while the antiviral response was not altered significantly.
We recommend the application of mouth wash solutions to COVID-19 patients, since our results indicate a reduction in infectivity and might govern the protection of health care professionals. Further improvement to the over the counter formulation can be made by utilizing zinc and dexpanthenol, as they which might be beneficial for the patients' health.
本研究旨在确定市售漱口液在降低 COVID-19 患者唾液中病毒载量从而降低其传染性方面的适用性。此外，还研究了新的实验性漱口液在可能的积极免疫调节方面的作用，这可能为积极的药物治疗效果提供额外的机会。
通过对 34 名住院 COVID-19 患者进行咽拭子 RT-qPCR 测量，在漱口前后采集咽拭子，确定漱口液的效果。通过病毒感染鼻咽上皮细胞的体外模型测定实验溶液的炎症调节作用。
临床初步研究表明，漱口液能够使大多数患者的唾液中的病毒载量降低约 90%。这种减少被确定持续约 6 小时。在实验溶液中，成分泛醇和锌能够降低细胞培养模型中促炎细胞因子的表达，而抗病毒反应没有明显改变。
我们建议将漱口液应用于 COVID-19 患者，因为我们的结果表明感染性降低，并可能保护医护人员的健康。通过利用锌和泛醇进一步改进非处方配方，可能对患者的健康有益。
