Department of Electrical and Computer Engineering, Sungkyunkwan University, Suwon, Republic of Korea; Electrical Engineering Graduate Program and itt CHIP - Semiconductor Institute, Universidade do Vale do Rio dos Sinos, São Leopoldo, Brazil.
Department of Electrical and Computer Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
Biosens Bioelectron. 2020 Jun 1;157:112167. doi: 10.1016/j.bios.2020.112167. Epub 2020 Mar 22.
Graphene-based transistors are promising devices in the evaluation of carrier density in biological analytes. We report on the design and fabrication of a graphene-based field-effect transistor for monitoring and assessing the interaction between the coagulation factors based on the charge carrier density in a blood sample. When biochemical reactions occurred during the coagulation cascade process, a dopant effect was noticed on the graphene surface by the change in Dirac point voltage values. Additional experiments were performed using blood samples treated with activators (vitamin K, calcium chloride, and thromboplastin reagent) and inhibitors (heparin drugs) to evaluate the selectivity of the graphene field-effect transistor devices. Since the transfer characteristic curves presented divergent behaviours for different levels of procoagulants and anticoagulants, the measurements showed that the devices can assess changes in the concentrations of factors that inhibit or accelerate the cascade process when using untreated and treated samples. Reproducibility was verified by testing samples from different sources. To the best of our knowledge, this study is the first to demonstrate the potential of graphene in monitoring the hemostasis process through the analysis of the electrical properties of human whole blood.
基于石墨烯的晶体管在评估生物分析物中的载流子密度方面是很有前途的器件。我们报告了一种基于石墨烯的场效应晶体管的设计和制造，用于监测和评估基于血液样本中电荷载流子密度的凝血因子之间的相互作用。当在凝血级联过程中发生生化反应时，通过观察到的狄拉克点电压值的变化，在石墨烯表面上注意到了掺杂剂效应。使用经过激活剂（维生素 K、氯化钙和凝血酶试剂）和抑制剂（肝素药物）处理的血液样本进行了额外的实验，以评估石墨烯场效应晶体管器件的选择性。由于转移特性曲线呈现出不同水平的促凝剂和抗凝剂的不同行为，测量结果表明，当使用未处理和处理的样本时，这些设备可以评估抑制或加速级联过程的因子浓度的变化。通过测试来自不同来源的样本，验证了重现性。据我们所知，这项研究首次通过分析人类全血的电特性，展示了石墨烯在监测止血过程中的潜力。
