Gwanggyo R&D Center, Medytox Inc., Suwon-si, Republic of Korea.
Osong R&D Center, Medytox Inc., Cheonju-si, Republic of Korea.
Dermatol Surg. 2020 Dec;46(12):e132-e138. doi: 10.1097/DSS.0000000000002402.
A new complexing protein-free botulinum toxin Type A (CBoNT) with the same mechanism of action as the botulinum toxin complex onabotulinumtoxinA (OBoNT) and complexing protein-free incobotulinumtoxinA (IBoNT) was recently developed.
To compare the local paresis and chemodenervation efficacy of 3 different botulinum toxin Type A preparations in mice.
Efficacy and duration of action of CBoNT, OBoNT, and IBoNT after a single intramuscular injection to the right gastrocnemius was evaluated by digit abduction score (DAS) and compound muscle action potential (CMAP) assays.
Mouse DAS and CMAP responses were comparable between CBoNT and OBoNT, indicating similar paresis and chemodenervation efficacy, as well as duration of action. Both botulinum toxins showed significantly higher efficacy and longer duration of action than IBoNT. Similarly, mean DAS potency of CBoNT (ED50: 3.85 ± 0.34 U/kg) and OBoNT (ED50: 4.13 ± 0.07 U/kg) were significantly higher compared with IBoNT (ED50: 6.70 ± 0.83 U/kg).
CBoNT displays the same efficacy as OBoNT as shown by their comparable chemodenervation and local paretic effects, and demonstrates superior efficacy and duration of action compared with IBoNT. Likewise, CBoNT has comparable DAS potency to OBoNT and is superior to IBoNT.
最近开发了一种与肉毒毒素复合物 onabotulinumtoxinA（OBoNT）和无复合物的 incobotulinumtoxinA（IBoNT）具有相同作用机制的新型无复合蛋白肉毒毒素 A 型（CBoNT）。
比较 3 种不同肉毒毒素 A 制剂在小鼠中的局部瘫痪和化学去神经作用效果。
通过数字外展评分（DAS）和复合肌肉动作电位（CMAP）测定，评估 CBoNT、OBoNT 和 IBoNT 单次肌肉内注射右侧比目鱼肌后的疗效和作用持续时间。
CBoNT 和 OBoNT 的小鼠 DAS 和 CMAP 反应相当，表明瘫痪和化学去神经作用效果以及作用持续时间相似。两种肉毒毒素的疗效均明显高于 IBoNT，作用持续时间也更长。同样，CBoNT（ED50：3.85±0.34 U/kg）和 OBoNT（ED50：4.13±0.07 U/kg）的平均 DAS 效价也明显高于 IBoNT（ED50：6.70±0.83 U/kg）。
CBoNT 显示出与 OBoNT 相同的疗效，其化学去神经和局部瘫痪作用相当，与 IBoNT 相比，其疗效和作用持续时间更优。同样，CBoNT 的 DAS 效价与 OBoNT 相当，优于 IBoNT。
