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海湾扇贝 Argopecten irradians 的染色体水平基因组组装。

Chromosome-level genome assembly of the bay scallop Argopecten irradians.

机构信息

School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, 11794-5000, USA.

Cornell Cooperative Extension of Suffolk County, Southold, NY, 11971, USA.

出版信息

Sci Data. 2024 Sep 28;11(1):1057. doi: 10.1038/s41597-024-03904-x.

Abstract

The bay scallop, Argopecten irradians, is a species of major commercial, cultural, and ecological importance. It is endemic to the eastern coast of the United States, but has also been introduced to China, where it supports a significant aquaculture industry. Here, we provide an annotated chromosome-level reference genome assembly for the bay scallop, assembled using PacBio and Hi-C data. The total genome size is 845.9 Mb, distributed over 1,503 scaffolds with a scaffold N50 of 44.3 Mb. The majority (92.9%) of the assembled genome is contained within the 16 largest scaffolds, corresponding to the 16 chromosomes confirmed by Hi-C analysis. The assembly also includes the complete mitochondrial genome. Approximately 36.2% of the genome consists of repetitive elements. The BUSCO analysis showed a completeness of 96.2%. We identified 33,772 protein-coding genes. This genome assembly will be a valuable resource for future research on evolutionary dynamics, adaptive mechanisms, and will support genome-assisted breeding, contributing to the conservation and management of this iconic species in the face of environmental and pathogenic challenges.

摘要

海湾扇贝(Argopecten irradians)是一种具有重要商业、文化和生态意义的物种。它原产于美国东海岸,但也已被引入中国,在中国支持着重要的水产养殖业。在这里,我们提供了一个海湾扇贝的注释染色体水平参考基因组组装,该组装使用了 PacBio 和 Hi-C 数据。总基因组大小为 845.9Mb,分布在 1503 个支架上,支架 N50 为 44.3Mb。组装的基因组大部分(92.9%)包含在 16 个最大的支架中,这些支架对应于 Hi-C 分析确定的 16 条染色体。该组装还包括完整的线粒体基因组。大约 36.2%的基因组由重复元件组成。BUSCO 分析显示其完整性为 96.2%。我们鉴定了 33772 个蛋白质编码基因。这个基因组组装将成为未来进化动态、适应机制研究的宝贵资源,并将支持基因组辅助育种,为应对环境和致病性挑战保护和管理这一标志性物种做出贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb75/11439060/0cafc58a5e76/41597_2024_3904_Fig1_HTML.jpg

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