Suppr超能文献

(Spreng.)Taub.提取物对……的体外抗真菌活性

In Vitro Antifungal Activity of (Spreng.) Taub. extracts against .

作者信息

Di Ciaccio Lucía S, Catalano Alejandra V, López Paula G, Rojas Dante, Cristos Diego, Fortunato Renée H, Salvat Adriana E

机构信息

Instituto de Patobiología Veterinaria, (IPvet), CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Prov. de Buenos Aires, Argentina.

Consejo Nacional de Investigaciones Científicas y Técnicas-CONICET, Ciudad Autónoma de Buenos Aires 1425, Argentina.

出版信息

Plants (Basel). 2020 Apr 2;9(4):438. doi: 10.3390/plants9040438.

Abstract

is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of , a species from northern Argentina (Oriental Chaco), against . The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 µg/mL, and the differences between the treatments and the inoculum control were 11 mm of and 10 mm of in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein.

摘要

是一种丝状腐生真菌,其定殖主要发生在收获后的谷物和油籽中。在某些条件下,它会产生称为黄曲霉毒素的霉菌毒素,这是一种强大的人类肝脏致癌物。本研究的目的是描述来自阿根廷北部(东方查科)的一种提取物对的抗真菌活性。报告了不同采集地点的抗真菌活性。提取物的最低抑菌浓度为125 µg/mL,处理组与接种物对照组在菌落生长方面的差异为,的差异为11 mm,的差异为10 mm。此外,用伊文思蓝染色处理的菌丝显示出膜和/或细胞壁的改变,使染料能够进入。进行了生物导向分级分离、高效液相色谱二极管阵列紫外/可见光(HPLC UV/VIS DAD)和超高效液相色谱电喷雾电离质谱(UPLC ESI-MS)分析,以表征提取物及其活性成分。HPLC UV/VIS DAD分析可以确定黄酮类化合物(黄酮醇和黄酮)、香豆素、萜类化合物和甾体的存在。活性成分的UPLC ESI/MS分析显示存在山奈酚、芹菜素、柚皮素、白杨素和大豆苷元。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8568/7238424/93c48b3ca559/plants-09-00438-g001.jpg

相似文献

1
In Vitro Antifungal Activity of (Spreng.) Taub. extracts against .
Plants (Basel). 2020 Apr 2;9(4):438. doi: 10.3390/plants9040438.
2
Antifungal activity of Parastrephia quadrangularis (Meyen) Cabrera extracts against Fusarium verticillioides.
Lett Appl Microbiol. 2018 Mar;66(3):244-251. doi: 10.1111/lam.12844. Epub 2018 Feb 7.
4
Green extraction of polyphenols from citrus peel by-products and their antifungal activity against .
Food Chem X. 2021 Oct 25;12:100144. doi: 10.1016/j.fochx.2021.100144. eCollection 2021 Dec 30.
6
Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry.
J Pharm Biomed Anal. 2006 Sep 26;42(3):354-61. doi: 10.1016/j.jpba.2006.04.017. Epub 2006 Jun 9.
10
RRLC-DAD-ESI-MS based and bioactivity guided phytochemical analysis and separation of coumarins from raw extracts of Trigonostemon lutescens.
J Pharm Biomed Anal. 2019 May 30;169:293-302. doi: 10.1016/j.jpba.2019.02.045. Epub 2019 Mar 13.

引用本文的文献

2
Natamycin Has an Inhibitory Effect on , the Pathogen of Chestnuts.
Molecules. 2023 Apr 25;28(9):3707. doi: 10.3390/molecules28093707.
3
Antifungal activity of the volatile organic compounds produced by strains WSJK-1 and Mby.
Front Microbiol. 2022 Oct 20;13:1034939. doi: 10.3389/fmicb.2022.1034939. eCollection 2022.
4
5
Plant-Derived Chemicals: A Source of Inspiration for New Drugs.
Plants (Basel). 2020 Nov 13;9(11):1562. doi: 10.3390/plants9111562.

本文引用的文献

2
Chemical Constituents of the Leaves of and Their Bioactivity.
Molecules. 2019 Jan 10;24(2):240. doi: 10.3390/molecules24020240.
4
THE USE OF PLANTS TO PROTECT PLANTS AND FOOD AGAINST FUNGAL PATHOGENS: A REVIEW.
Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):120-127. doi: 10.21010/ajtcam.v14i4.14. eCollection 2017.
5
The antifungal pipeline: a reality check.
Nat Rev Drug Discov. 2017 Sep;16(9):603-616. doi: 10.1038/nrd.2017.46. Epub 2017 May 12.
6
Appraisal of phytochemical and in vitro biological attributes of an unexplored folklore: Rhus Punjabensis Stewart.
BMC Complement Altern Med. 2017 Mar 9;17(1):146. doi: 10.1186/s12906-017-1659-6.
7
A Concise History of Mycotoxin Research.
J Agric Food Chem. 2017 Aug 23;65(33):7021-7033. doi: 10.1021/acs.jafc.6b04494. Epub 2016 Dec 27.
8
"Is there an emerging need for new antifungals?".
Expert Opin Emerg Drugs. 2016 Jun;21(2):129-31. doi: 10.1517/14728214.2016.1155554. Epub 2016 Mar 7.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验