Di Ciaccio Lucía S, Catalano Alejandra V, López Paula G, Rojas Dante, Cristos Diego, Fortunato Renée H, Salvat Adriana E
Instituto de Patobiología Veterinaria, (IPvet), CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Prov. de Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas-CONICET, Ciudad Autónoma de Buenos Aires 1425, Argentina.
Plants (Basel). 2020 Apr 2;9(4):438. doi: 10.3390/plants9040438.
is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of , a species from northern Argentina (Oriental Chaco), against . The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 µg/mL, and the differences between the treatments and the inoculum control were 11 mm of and 10 mm of in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein.
是一种丝状腐生真菌,其定殖主要发生在收获后的谷物和油籽中。在某些条件下,它会产生称为黄曲霉毒素的霉菌毒素,这是一种强大的人类肝脏致癌物。本研究的目的是描述来自阿根廷北部(东方查科)的一种提取物对的抗真菌活性。报告了不同采集地点的抗真菌活性。提取物的最低抑菌浓度为125 µg/mL,处理组与接种物对照组在菌落生长方面的差异为,的差异为11 mm,的差异为10 mm。此外,用伊文思蓝染色处理的菌丝显示出膜和/或细胞壁的改变,使染料能够进入。进行了生物导向分级分离、高效液相色谱二极管阵列紫外/可见光(HPLC UV/VIS DAD)和超高效液相色谱电喷雾电离质谱(UPLC ESI-MS)分析,以表征提取物及其活性成分。HPLC UV/VIS DAD分析可以确定黄酮类化合物(黄酮醇和黄酮)、香豆素、萜类化合物和甾体的存在。活性成分的UPLC ESI/MS分析显示存在山奈酚、芹菜素、柚皮素、白杨素和大豆苷元。