Khoshfarman-Borji H, Pahlavan Yali M, Bozorg-Amirkalaee M
Department of Plant Protection, Faculty of Agriculture, Shahid Bahonar University, Iran.
Department of Plant Protection, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Iran.
Bull Entomol Res. 2020 Oct;110(5):597-610. doi: 10.1017/S0007485320000097. Epub 2020 Apr 7.
The cabbage aphid, Brevicoryne brassicae L. (Hem: Aphididae), is one of the most serious pests of canola worldwide. In this research, the effects of Pseudomonas putida, salicylic acid (SA), and integrated application of both inducers were studied on the resistance of canola to B. brassicae. In free-choice situation, the number of B. brassicae attracted on canola plants under treatments containing P. putida and SA was significantly lower compared to control plants. In the life table study, pre-adult survival, longevity, reproductive period, and fecundity of this aphid were lowest on plants treated with P. putida + SA. The net reproductive rate (R0), intrinsic rate of population increase (r), and finite rate of increase (λ) of B. brassicae decreased significantly in the following order: control (47.19 offspring, 0.293 and 1.340 day-1), P. putida (16.7 offspring, 0.238 and 1.269 day-1), SA (6.37 offspring, 0.163 and 1.178 day-1), and P. putida + SA (3.24 offspring, 0.112 and 1.119 day-1). Moreover, the beneficial effect of the integrated application of P. putida and SA on plant growth parameters was significantly evident in our study. The highest values of glucosinolates, total phenol, and flavonoids were recorded in P. putida + SA treatment. We concluded that canola plants treated with P. putida + SA are more resistant to the cabbage aphid. These findings demonstrated that SA integrated with P. putida on canola plants act effectively for reducing the population of B. brassicae and can be used in integrated management programs of this pest.
甘蓝蚜，Brevicoryne brassicae L.（半翅目：蚜科），是全球油菜最严重的害虫之一。本研究中，研究了恶臭假单胞菌、水杨酸（SA）以及两者诱导剂的综合应用对油菜抗甘蓝蚜的影响。在自由选择情况下，与对照植株相比，含有恶臭假单胞菌和SA处理的油菜植株上吸引的甘蓝蚜数量显著减少。在生命表研究中，该蚜虫的未成熟若虫存活率、寿命、繁殖期和繁殖力在恶臭假单胞菌+SA处理的植株上最低。甘蓝蚜的净繁殖率（R0）、种群内禀增长率（r）和周限增长率（λ）显著降低，顺序如下：对照（47.19只后代，0.293和1.340天-1）、恶臭假单胞菌（16.7只后代，0.238和1.269天-1）、SA（6.37只后代，0.163和1.178天-1）、恶臭假单胞菌+SA（3.24只后代，0.112和1.119天-1）。此外，在我们的研究中，恶臭假单胞菌和SA综合应用对植物生长参数的有益影响显著。在恶臭假单胞菌+SA处理中，硫代葡萄糖苷、总酚和类黄酮的含量最高。我们得出结论，用恶臭假单胞菌+SA处理的油菜植株对甘蓝蚜更具抗性。这些发现表明，SA与恶臭假单胞菌在油菜植株上联合使用可有效减少甘蓝蚜种群数量，并可用于该害虫的综合管理方案。
