Sattari Nasab R, Pahlavan Yali M, Bozorg-Amirkalaee M
Department of Plant Protection, Faculty of Agriculture, Shahid Bahonar University, Kerman, Iran.
Department of Plant Protection, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
Bull Entomol Res. 2019 Aug;109(4):479-489. doi: 10.1017/S0007485318000779. Epub 2018 Oct 23.
The cabbage aphid, Brevicoryne brassicae L. (Hem: Aphididae), is an important pest of canola that can considerably limit profitable crop production either through direct feeding or via transmission of plant pathogenic viruses. One of the most effective approaches of pest control is the use of biostimulants. In this study, the effects of humic acid, plant growth-promoting rhizobacteria (PGPR), and integrated application of both compounds were investigated on life table parameters of B. brassicae, and the tolerance of canola to this pest. B. brassicae reared on plants treated with these compounds had the lower longevity, fecundity, and reproductive period compared with control treatment. The intrinsic rate of natural increase (r) and finite rate of increase (λ) were lowest on PGPR treatment (0.181 ± 0.004 day-1 and 1.198 ± 0.004 day-1, respectively) and highest on control (0.202 ± 0.005 day-1 and 1.224 ± 0.006 day-1, respectively). The net reproductive rate (R0) under treatments of humic acid, PGPR and humic acid + PGPR was lower than control. There was no significant difference in generation time (T) of B. brassicae among the tested treatments. In the tolerance test, plants treated with PGPR alone or in integrated with humic acid had the highest tolerance against B. brassicae. The highest values of total phenol, flavonoids, and glucosinolates were observed in treatments of PGPR and humic acid + PGPR. Basing on the antibiosis and tolerance analyses in this study, we concluded that canola plants treated with PGPR are more resistant to B. brassicae. These findings could be useful for integrated pest management of B. brassicae in canola fields.
甘蓝蚜（Brevicoryne brassicae L.，半翅目：蚜科）是油菜的一种重要害虫，它可通过直接取食或传播植物病原病毒，严重限制油菜的高产栽培。害虫防治最有效的方法之一是使用生物刺激素。本研究调查了腐殖酸、植物根际促生菌（PGPR）以及二者混合施用对甘蓝蚜生命表参数和油菜对该害虫耐受性的影响。与对照处理相比，在经这些化合物处理的植株上饲养的甘蓝蚜，其寿命、繁殖力和繁殖期均较短。在PGPR处理下，内禀增长率（r）和周限增长率（λ）最低（分别为0.181±0.004天-1和1.198±0.004天-1），而在对照处理下最高（分别为0.202±0.005天-1和1.224±0.006天-1）。腐殖酸、PGPR和腐殖酸+PGPR处理下的净生殖率（R0）均低于对照。在所测试的处理中，甘蓝蚜的世代历期（T）没有显著差异。在耐受性试验中，单独施用PGPR或与腐殖酸混合施用处理的植株对甘蓝蚜的耐受性最高。在PGPR和腐殖酸+PGPR处理中，总酚、类黄酮和芥子油苷的含量最高。基于本研究中的抗生性和耐受性分析，我们得出结论，用PGPR处理的油菜植株对甘蓝蚜具有更强的抗性。这些研究结果可能有助于油菜田甘蓝蚜的综合防治。
