Department of Pediatric Hematologist and Oncologist, Bahrami Childrenhospital, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Anal Chim Acta. 2020 May 1;1109:122-129. doi: 10.1016/j.aca.2020.02.060. Epub 2020 Feb 27.
Leukemia is the worst type of malignancy in children which its proper diagnosis can be used in the treatment. We design the turn-off sensor by using the different electrochemical patterns of liposomes to the detection of acute lymphoblastic leukemia cells. Our design is first sandwiched by lectin liposome which increases the electrochemical exchange on the electrode. With the addition of Molt-4 cells, the bonding connection between the n-glycan and lectin can also increase the electrochemical exchange with the high detection cells. Subsequently, the addition of boronic acid liposomes decreases the resistance due to covering glycosylation bond and the sensor is turn-off. But stable and specific binding with the sialic acid causes the higher detection of Molt-4 cells. The electrochemical measurements are performed between the potentials at -0.4 V and +0.4 V with 1 mM [Fe(CN)] . So, for the first time, we designed a cells sensor based on the different patterns of liposomes to screening the N-glycan cells, which can be used in the point of care tests with higher sensitivity.
白血病是儿童中最严重的恶性肿瘤，其正确诊断可用于治疗。我们设计了关闭传感器，利用脂质体的不同电化学模式来检测急性淋巴细胞白血病细胞。我们的设计首先被凝集素脂质体夹在中间，这增加了电极上的电化学交换。随着 Molt-4 细胞的加入，n-聚糖和凝集素之间的结合连接也可以增加与高检测细胞的电化学交换。随后，加入硼酸脂质体由于覆盖糖基化键而降低电阻，传感器关闭。但是与唾液酸的稳定和特异性结合导致对 Molt-4 细胞的更高检测。电化学测量在-0.4 V 和+0.4 V 之间进行，使用 1 mM [Fe(CN)] 。因此，我们首次设计了一种基于脂质体不同模式的细胞传感器，用于筛选 N-聚糖细胞，可用于具有更高灵敏度的即时检测。
