Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Sci Rep. 2021 Nov 26;11(1):22969. doi: 10.1038/s41598-021-02029-0.
It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1 receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)] probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au/Antibody-SARS-CoV-2 on the MV-gal1@SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection).For the first time, our sensor has the capacity of screening of any spike virus, and the specific detection of COVID-19 (LOD: 4.57 × 10 copies/mL) by using the natural bed-receptor and a specific antibody in the point of care test.
在病毒筛查过程中，我们必须使用能够检测病毒特异性的方法。在这项研究中，我们使用双平台依次识别任何 Spike 病毒和特定的 SARS-CoV-2 抗原。我们引入天然床受体表面作为带有聚糖结合能力的微囊泡半乳糖素 1（MV-gal1），以筛选每一种 Spike 病毒。MV 是具有 gal-1 受体的天然囊泡。Gal-1 是一种可以结合聚糖的凝集素受体。将 MV-gal1 滴加到 SCPE/GNP 上后，由于与 [Fe(CN)]探针的电化学交换增加，传感器被开启。滴加 SARS-CoV-2 的病毒颗粒会覆盖糖键，从而关闭传感器（早期筛查）。然后，通过在 MV-gal1@SARS-CoV-2 抗原上添加 Au/抗 SARS-CoV-2 抗体，由于 AuNP 的电化学放大作用，传感器再次开启（特异性检测）。我们的传感器首次具有筛查任何 Spike 病毒的能力，并通过在即时检测中使用天然床受体和特异性抗体，实现了对 COVID-19 的特异性检测（LOD：4.57×10 拷贝/mL）。
