一项合作研究：高效液相色谱法测定动物饲料中的卡巴氧和酒石酸噻嘧啶。

A collaborative study: high-pressure liquid chromatographic determination of carbadox and pyrantel tartrate in animal feeds.

作者信息

Thorpe V A

机构信息

Michigan Department of Agriculture, Laboratory Division, East Lansing 48823.

出版信息

J Chromatogr Sci. 1988 Nov;26(11):545-50. doi: 10.1093/chromsci/26.11.545.

DOI:10.1093/chromsci/26.11.545

PMID:3225304

Abstract

Carbadox (CBX), an antibacterial agent, and pyrantel tartrate (PT), an anthelmintic, are formulated either separately or together in swine feeds. The official Association of Official Analytical Chemists (AOAC) spectrophotometric methods for both drugs are long, nonspecific, and require standard addition techniques. Results by this technique are positively biased. A simple, direct, specific, high-pressure liquid chromatographic (HPLC) method to determine either one or both drugs simultaneously with apparent accuracy and precision is developed. Drugs are released from feed matrices by water, extracted with dimethylformamide (DMF), cleaned up on alumina, and quantitated by direct comparison to standards using a Whatman Partisil 10 ODS-3 column and a mobile solvent containing 23.5 +/- 1.5% DMF in phosphate buffer (pH 2.0). Fourteen laboratories participated in a collaborative study of this method for determination of CBX and PT in animal feeds.

摘要

卡巴多司（CBX）是一种抗菌剂，而酒石酸噻嘧啶（PT）是一种驱虫药，它们可以单独或一起添加到猪饲料中。美国官方分析化学家协会（AOAC）针对这两种药物的分光光度法耗时较长、缺乏特异性，且需要采用标准加入技术。该技术得到的结果存在正偏差。本文开发了一种简单、直接、特异的高效液相色谱（HPLC）方法，可同时准确、精密地测定其中一种或两种药物。药物通过水从饲料基质中释放出来，用二甲基甲酰胺（DMF）萃取，在氧化铝上进行净化处理，并使用沃特曼Partisil 10 ODS-3色谱柱和含23.5±1.5% DMF的磷酸盐缓冲液（pH 2.0）作为流动相溶剂，通过与标准品直接比较进行定量分析。十四个实验室参与了该方法测定动物饲料中CBX和PT的协同研究。