饲料中卡巴多司和喹乙醇的柱液相色谱测定法
Column liquid chromatographic determination of carbadox and olaquindox in feeds.
作者信息
dos Ramos F J, da Silveira I N, de Graaf G
机构信息
Laboratório de Bromatologia e Nutrição, Faculdade de Farmácia da Universidade de Coimbra, Portugal.
出版信息
J Chromatogr. 1991 Sep 27;558(1):125-30. doi: 10.1016/0021-9673(91)80117-y.
A column liquid chromatographic method for simultaneous determination of carbadox and olaquindox in swine feeds is described. The drugs were extracted from feeds with carbon tetrachloride-dimethylformamide (80:20) at 60 degrees C for 30 min. The extract was mixed with water (25:45). After centrifugation the aqueous layer was chromatographed on a reversed-phase column using gradient elution and ultraviolet detection at wavelengths of 305 and 262 nm. Recoveries from samples fortified at levels of 20-50 ppm were 92 +/- 9% for carbadox and 93 +/- 6% for olaquindox (means +/- standard deviations, n = 71).
描述了一种同时测定猪饲料中卡巴氧和喹乙醇的柱液相色谱法。药物在60℃下用四氯化碳 - 二甲基甲酰胺（80:20）从饲料中提取30分钟。提取物与水（25:45）混合。离心后，水层在反相柱上进行色谱分析，采用梯度洗脱，并在305和262nm波长处进行紫外检测。在20 - 50ppm加标水平的样品中，卡巴氧的回收率为92±9%，喹乙醇的回收率为93±6%（平均值±标准差，n = 71）。
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