Department of Dermatology, Zhongshan Hospital Fudan University (Xiamen Branch), Xiamen, China.
Department of Dermatology, Zhongshan Hospital Fudan University, Shanghai, China.
Antimicrob Agents Chemother. 2020 May 21;64(6). doi: 10.1128/AAC.00290-20.
and interactions of minocycline and azoles, including itraconazole, voriconazole, and posaconazole, against filamentous pathogenic fungi were investigated. A total of 56 clinical isolates were studied via broth microdilution checkerboard technique, including 20 strains of , 7 strains of , 16 strains of , 10 strains of , and 3 strain s of The results revealed that minocycline did not exhibit any significant antifungal activity against any of the tested strains. However, favorable synergy of minocycline with itraconazole, voriconazole, or posaconazole was observed against 34 (61%), 28 (50%), and 38 (68%) isolates, respectively, including azole-resistant and spp. with inherently high MICs of azoles. Synergistic combinations resulted in 4-fold to 16-fold reduction of effective MICs of minocycline and azoles. No antagonism was observed. effects of minocycline-azole combinations were evaluated by survival assay in a model infected with strain BMU00034; strain FS9; and strains AF293, AFR1, and AFR2. Minocycline acted synergistically with azoles and significantly increased larvae survival in all isolates ( < 0.001), including azole-resistant and azole-inactive spp. In conclusion, the results suggested that minocycline combined with azoles may help to enhance the antifungal susceptibilities of azoles against pathogenic fungi and had the potential to overcome azole resistance issues.
研究了米诺环素与唑类药物（包括伊曲康唑、伏立康唑和泊沙康唑）的相互作用，以对抗丝状致病真菌。通过肉汤微量稀释棋盘技术研究了总共 56 株临床分离株，包括 20 株 ，7 株 ，16 株 ，10 株 ，和 3 株 。结果表明，米诺环素对所有测试菌株均无明显的抗真菌活性。然而，米诺环素与伊曲康唑、伏立康唑或泊沙康唑联合使用对 34（61%）、28（50%）和 38（68%）分离株表现出良好的协同作用，包括具有固有高唑类药物 MIC 的唑类耐药 和 种。协同组合导致米诺环素和唑类药物的有效 MIC 降低了 4 倍至 16 倍。未观察到拮抗作用。通过感染 菌株 BMU00034、 菌株 FS9 和 菌株 AF293、AFR1 和 AFR2 的 模型评估了米诺环素-唑类药物组合的 作用。米诺环素与唑类药物协同作用，显著增加了所有分离株（ < 0.001）的幼虫存活率，包括唑类耐药 和唑类非活性 种。总之，结果表明米诺环素与唑类药物联合使用可能有助于增强唑类药物对致病性真菌的抗真菌敏感性，并有可能克服唑类药物耐药问题。
