Kurnaz Recep, Balta Orhan
Department of Orthopaedics and Traumatology, Eskişehir Acıbadem Hospital, Eskişehir, Turkey.
Department of Orthopaedics and Traumatology, Gaziosmanpaşa University, School of Medicine, Tokat, Turkey.
Acta Orthop Traumatol Turc. 2020 Mar;54(2):186-195. doi: 10.5152/j.aott.2020.02.20.
This study aimed to investigate the effect of platelet-rich plasma (PRP) and platelet-rich fibrin matrix (PRFM) on the healing of vertical medial meniscal tears in a rabbit model.
The study was conducted on 72 New Zealand mature rabbits aged more than 6 months. Rabbits were randomly assigned to six groups: control (C) group, meniscal repair (MR) group, PRP group, PRFM group, MR+PRP group, and MR+PRFM group, with 12 rabbits in each group. A 5-mm full-thickness vertical tear was created in the avascular zone of the medial meniscus corpus in the right knee of all rabbits. The respective treatment for each group was given to the meniscal tear in each rabbit. Histological evaluation of healing was performed 6 and 12 weeks after surgery.
Defect filling and collagen formation remained low in the C group. However, in all other groups, there was no significant difference in the 6th week. In the 12th week, similar results as those obtained in the 6th week were obtained. In the C group, there was a difference in defect filling and cell type. This difference was that the defect filling and collagen formation remained low in the 12th week. No significant difference was observed between MR, MR + PRP and MR + PRFM groups. The MR group significantly differed from the other groups in the defect-filling rate and cell type; however, the use of PRP and PRFM did not provide an obvious benefit.
The contradictory results obtained in previous studies emphasize the need for further research on the use of PRP in meniscal recovery and repair. We believe that if surgery is indicated, repair is absolutely necessary to improve the healing of the tissue in meniscal tears. Studies using human meniscal tissue for meniscal injury and those that evaluate clinical applications of PRP are warranted.
本研究旨在探讨富血小板血浆（PRP）和富血小板纤维蛋白基质（PRFM）对兔模型中垂直内侧半月板撕裂愈合的影响。
对72只6个月以上的新西兰成年兔进行研究。将兔子随机分为六组：对照组（C组）、半月板修复（MR）组、PRP组、PRFM组、MR+PRP组和MR+PRFM组，每组12只兔子。在所有兔子右膝内侧半月板体部的无血管区制造一个5毫米的全层垂直撕裂。对每组兔子的半月板撕裂进行相应治疗。在术后6周和12周进行愈合的组织学评估。
C组的缺损填充和胶原形成仍然较低。然而，在所有其他组中，第6周时没有显著差异。在第12周时，获得了与第6周相似的结果。在C组中，缺损填充和细胞类型存在差异。这种差异在于第12周时缺损填充和胶原形成仍然较低。MR组、MR+PRP组和MR+PRFM组之间未观察到显著差异。MR组在缺损填充率和细胞类型方面与其他组有显著差异；然而，使用PRP和PRFM并没有提供明显的益处。
先前研究中获得的矛盾结果强调了对PRP在半月板恢复和修复中的应用进行进一步研究的必要性。我们认为，如果需要手术，修复对于改善半月板撕裂组织的愈合绝对必要。有必要开展使用人半月板组织研究半月板损伤以及评估PRP临床应用的研究。
