Zhang Kefan, Zhang Chenchen, Ren Qiang, Wang Dening, Sun Li, Wang Xin, Wang Yaomin, Shi Hui
Department of Orthopedics and Sports Medicine, Binzhou Medical University Hospital, Binzhou Shandong, 256603, P. R. China.
Department of Operating Room, Binzhou Medical University Hospital, Binzhou Shandong, 256603, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2024 Feb 15;38(2):183-188. doi: 10.7507/1002-1892.202311075.
To explore the best centrifuge condition for preparing rabbit leukocyte-poor platelet-rich plasma (LP-PRP) by using single centrifugation method.
Sixteen healthy New Zealand rabbits, aged 3-4 months, were utilized in the investigation. A total of 15 mL anticoagulated blood was extracted from the central ear artery of each rabbit, with a repeat of the blood collection procedure after 1 and 2 months. The obtained blood specimens were individually subjected to centrifugation at a radius of 16.7 cm and speeds of 1 200, 1 300, 1 400, and 1 500 r/min (equivalent to centrifugal forces of 269× , 315× , 365× , and 420× ) for durations of 2, 3, 4, and 5 minutes, resulting in a total of 16 groups. Following centrifugation, collect plasma from each group to a distance of 1.5 mL from the separation plane. The volumes, platelet enrichment coefficient, and platelet recovery rates of LP-PRP in each group, under varying centrifugation conditions, were methodically computed and subsequently compared.
The volume of LP-PRP obtained under all centrifugation conditions ranged from 1.8 to 7.6 mL. At a consistent centrifugal speed, an extension of centrifugation time leaded to a significant increase in the volume of LP-PRP, accompanied by a declining trend in the platelet enrichment coefficient of LP-PRP. When centrifuged for 2 minutes, the volume of LP-PRP at speeds of 1 200 and 1 300 r/min was less than 2.0 mL, while the volume of LP-PRP obtained under other conditions was more than 2.0 mL. When centrifuged for 4 and 5 minutes, the volume of LP-PRP obtained at each speed was more than 4 mL. LP-PRP with a platelet enrichment coefficient more than 2.0 could be prepared by centrifuging at 1 200 r/min for each time group and 1 300 r/min for 2 and 3 minutes, and the highest LP-PRP platelet enrichment coefficient could be obtained by centrifugation for 2 minutes at a speed of 1 200 r/min. The platelet recovery rates of LP-PRP obtained by centrifugation at 1 200 r/min for 4 and 5 minutes, as well as centrifugation at 1 400 r/min for 5 minutes, were both greater than 60%. There was no significant difference between the groups when centrifuged at 1 200 r/min for 4 and 5 minutes ( >0.05).
In the process of preparing rabbit LP-PRP using a single centrifugation method, collecting 15 mL of blood and centrifuging at a radius of 16.7 cm and speed of 1 200 r/min for 4 minutes can prepare LP-PRP with a volume exceeding 2.0 mL, platelet enrichment coefficient exceeding 2.0, and platelet recovery rate exceeding 60%. This centrifugal condition can achieve the optimal LP-PRP action parameters in the shortest possible time.
采用单次离心法探索制备兔少白细胞富血小板血浆（LP-PRP）的最佳离心条件。
选取16只3 - 4月龄健康新西兰兔用于本研究。从每只兔的耳中央动脉抽取15 mL抗凝血液，1个月和2个月后重复采血过程。将采集到的血液标本分别在半径为16.7 cm、转速为1200、1300、1400和1500 r/min（相当于离心力269×、315×、365×和420×）的条件下离心2、3、4和5分钟，共形成16组。离心后，从每组中收集距分离平面1.5 mL的血浆。系统计算并比较不同离心条件下每组LP-PRP的体积、血小板富集系数和血小板回收率。
所有离心条件下获得的LP-PRP体积在1.8至7.6 mL之间。在离心速度一致时，延长离心时间会使LP-PRP体积显著增加，同时LP-PRP的血小板富集系数呈下降趋势。离心2分钟时，1200和1300 r/min转速下的LP-PRP体积小于2.0 mL，其他条件下获得的LP-PRP体积大于2.0 mL。离心4分钟和5分钟时，各转速下获得的LP-PRP体积均大于4 mL。每次离心组在1200 r/min离心以及在1300 r/min离心2分钟和3分钟时可制备出血小板富集系数大于2.0的LP-PRP，1200 r/min离心2分钟可获得最高的LP-PRP血小板富集系数。1200 r/min离心4分钟和5分钟以及1400 r/min离心5分钟获得的LP-PRP血小板回收率均大于60%。120 r/min离心4分钟和5分钟时各组间差异无统计学意义（>0.05）。
采用单次离心法制备兔LP-PRP的过程中，采集15 mL血液，在半径为16.7 cm、转速为1200 r/min条件下离心4分钟，可制备出体积超过2.0 mL、血小板富集系数超过2.0且血小板回收率超过60%的LP-PRP。该离心条件能在最短时间内实现LP-PRP的最佳作用参数。
