Yu Xia, Song Hongliang, Huang Jing, Chen Yongxuan, Dai Ming, Lin Xucong, Xie Zenghong
Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 350108, China.
J Mater Chem B. 2018 Apr 7;6(13):1965-1972. doi: 10.1039/c7tb03319b. Epub 2018 Mar 16.
A novel hybrid affinity monolith with a high aptamer coverage density was developed via a highly efficient modification of aptamer@gold nanoparticles (Apt@AuNPs) on a well-controlled 3D skeletal POSS-polyethylenimine (PEI) matrix. The effects of amine reagents and the skeletal structures of the hybrid monoliths on the modification efficiency of Apt@AuNPs were studied, and the resultant Apt@AuNPs@POSS-PEI1200 monolith was proposed with a high coverage density of aptamer of up to 1413 pmol μL, which is 4-5 times more than the coverage density when modified with Apt@AuNPs that was reported previously. Characterization including morphology, spectra analysis, mechanical stability, binding capacity and specificity studies was also carried out. Significant specific recognition targeting of ochratoxin A (OTA) was achieved with a detection limit as low as 0.06 ng mL. High recoveries of OTA were achieved of 92.6 ± 1.3% to 94.7 ± 1.4% (n = 3), while the cross-reactivity towards analogues such as ochratoxin B (OTB) and aflatoxin B (AFB) was weak. This highlights a facile approach to POSS-based affinity monoliths with a high aptamer coverage density for sensitive and selective recognition of a trace target (OTA).
通过在可控的三维骨架倍半硅氧烷 - 聚乙烯亚胺（POSS-PEI）基质上高效修饰适配体@金纳米颗粒（Apt@AuNPs），开发了一种具有高适配体覆盖密度的新型混合亲和整体柱。研究了胺试剂和混合整体柱的骨架结构对Apt@AuNPs修饰效率的影响，并提出了所得的Apt@AuNPs@POSS-PEI1200整体柱，其适配体覆盖密度高达1413 pmol μL，比先前报道的用Apt@AuNPs修饰时的覆盖密度高4至5倍。还进行了包括形态、光谱分析、机械稳定性、结合能力和特异性研究在内的表征。实现了对赭曲霉毒素A（OTA）的显著特异性识别，检测限低至0.06 ng mL。OTA的回收率很高，为92.6±1.3%至94.7±1.4%（n = 3），而对赭曲霉毒素B（OTB）和黄曲霉毒素B（AFB）等类似物的交叉反应较弱。这突出了一种简便的方法来制备具有高适配体覆盖密度的基于POSS的亲和整体柱，用于灵敏且选择性地识别痕量目标物（OTA）。
