Xu Jinhua, Liu Ting, Chi Jinxin, Zhang Wen, Lin Chenchen, Lin Xucong, Xie Zenghong
Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 350108, China.
Xiamen Huaxia University, Xiamen, 361024, China.
Talanta. 2020 Nov 1;219:121309. doi: 10.1016/j.talanta.2020.121309. Epub 2020 Jun 27.
Via the facile photopolymerization and thiol-ene click chemistry, a hydrophilic polyhedral oligomeric silsesquioxane (POSS)-containing affinity monolithic column with a high load of aptamers and fast preparation was presented, and successfully applied to the efficient specific recognition and sensitive detection of zearalenone (ZEN) by coupling with HPLC. Optimization of polymerization reaction and polymer recipe were studied, and characteristics such as structural morphology, affinity performance and specificity to ZEN were evaluated. A highly hydrophilic POSS-based aptamer affinity monolith was rapidly prepared in 1.1 h, and a high capacity of aptamer reached 2772.61 pmol/μL that was much higher than that of the current POSS-based aptamer affinity monoliths. By this way, the hydrophilic nature and aptamer coverage indensity in POSS monoliths were well improved. The mixed-mode mechanism including aptamer affinity recognition and hydrophilic interaction were employed for the specific analysis of ZEN. Applied to the monitoring of ZEN, a good selective recognition and sensitive detection were obtained with the detection limit (LOD) of 0.02 ng/mL. The stability and reproducibility were acceptable and the intra-day, inter-day and column-to-column relative standard deviations (RSDs) of the ZEN recovery were gained in the range from 2.28% to 5.32%, respectively. Satisfactory recoveries of ZEN in rice samples were realized ad 98.6 ± 2.8%-100.8 ± 2.0%. It might provide a preferable access to online specific analysis of ZEN via the hydrophilic POSS-based affinity monolith with a large capacity of aptamers and a high preparation efficiency.
通过简便的光聚合反应和硫醇-烯点击化学，制备了一种负载量高且制备快速的含亲水性多面体低聚倍半硅氧烷（POSS）的亲和整体柱，并通过与高效液相色谱联用成功应用于玉米赤霉烯酮（ZEN）的高效特异性识别和灵敏检测。研究了聚合反应和聚合物配方的优化，并对其结构形态、亲和性能和对ZEN的特异性等特性进行了评估。在1.1小时内快速制备了一种高度亲水性的基于POSS的适配体亲和整体柱，其适配体的高负载量达到2772.61 pmol/μL，远高于目前基于POSS的适配体亲和整体柱。通过这种方式，POSS整体柱的亲水性和适配体覆盖密度得到了很好的改善。采用包括适配体亲和识别和亲水相互作用的混合模式机制对ZEN进行特异性分析。应用于ZEN的监测，获得了良好的选择性识别和灵敏检测，检测限（LOD）为0.02 ng/mL。稳定性和重现性良好，ZEN回收率的日内、日间和柱间相对标准偏差（RSD）分别在2.28%至5.32%范围内。水稻样品中ZEN的回收率令人满意，为98.6±2.8% - 100.8±2.0%。它可能为通过具有高适配体负载量和高制备效率的亲水性基于POSS的亲和整体柱对ZEN进行在线特异性分析提供一种较好的途径。
